Penghambatan Proses Kematian Sel Neuron Menggunakan Camillia Sinensis Dengan Bahan Aktif Egcg Pada Rattus Norvegicus Model Stroke Iskemik Akut

• Main Author: Abdulloh Machin
• Format: Theses and Dissertations NonPeerReviewed
• Language: Indonesian; English; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian
• Published: 2020
• Subjects: RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
• Online Access: http://repository.unair.ac.id/102967/1/1.%20HALAMAN%20JUDUL.pdf; http://repository.unair.ac.id/102967/2/2.%20ABSTRACT.pdf; http://repository.unair.ac.id/102967/3/3.%20DAFTAR%20ISI.pdf; http://repository.unair.ac.id/102967/4/4.%20BAB%201%20PENDAHULUAN.pdf; http://repository.unair.ac.id/102967/5/5.%20BAB%202%20TINJAUAN%20PUSTAKA.pdf; http://repository.unair.ac.id/102967/6/6.%20BAB%203%20KERANGKA%20KONSEPTUAL%20DAN%20HIPOTESIS%20PENELITIAN.pdf; http://repository.unair.ac.id/102967/7/7.%20BAB%204%20METODE%20PENELITIAN.pdf; http://repository.unair.ac.id/102967/8/8.%20BAB%205%20HASIL%20PENELITIAN.pdf; http://repository.unair.ac.id/102967/9/9.%20BAB%206%20PEMBAHASAN.pdf; http://repository.unair.ac.id/102967/10/10.%20BAB%207%20KESIMPULAN%20DAN%20SARAN.pdf; http://repository.unair.ac.id/102967/11/11.%20DAFTAR%20PUSTAKA.pdf; http://repository.unair.ac.id/102967/12/12.%20LAMPIRAN.pdf; http://repository.unair.ac.id/102967/13/PERMOHONAN%20EMBARGO.pdf; http://repository.unair.ac.id/102967/; http://www.lib.unair.ac.id
• Institution: Universitas Airlangga

Background: Stroke is the most prevalent neurological disorders in the world. During ischemic stroke there is increasing oxidative stres that will cause cell death through apoptosis and necroptosis pathways. Methods: We perform in vivo study using male Rattus Novergicus within 5 groups, control MCAO, EGCG 10 mg/kgBB, EGCG 20 mg/kgBB, EGCG 30 mg/kgBB, and extract green tea 30 mg/kgBB. Before performing MCAO models all study subject examine for Ladder Rung, and Y-Maze. We perform MCAO model using clamping carotid artery for 180 minutes. All groups are treated for 7 days and at day 7th we perform ladder rung and Y maze examination before research subject is sacrifice and examine HMGB1 using ELISA methods, and IHC for HO-1, TNFR1, RIP3, BCL-2 and Caspase-3. Result: There is significant different in all intervention group compared to control group on HO-1 (p<0,05). There is no significant different in all groups compared to control group in HMGB1. There is also significant different in all intervention group started at EGCG 20 mg/kgBW compared to control group on TNFR1 (p<0,05), significant different for RIP3 started at EGCG 20 mg/kgBW and extract green tea group (p<0,05), BCL-2 for all intervention group (p<0,05), Caspase-3 at EGCG 30mg/kgBW (p=0,004) and green tea extract group (p=0,019). There are no significant different on ladder rung at days 7th for all groups. There is also significant different in Y-Maze Score at green tea extract groups (p=0,048). There is significant correlation between HO-1 and BCL-2 (r=-0,655; p=0,000), BCL-2 and Caspase-3 (r=-0,5 ; p=0,000), Caspase-3 and Y-Maze (r=0,332; p=0,001), TNFR1 and RIP3 (r = 0,551; p=0,000) and we didn’t find correlation between HMGB1 and TNFR1 (r=0,029; p=0,838), RIP3 and Y-Maze (r=0,18; p=0,167). Conclusion: Green tea with its active coumpound EGCG can inhibit neuronal cell death through apoptosis and necroptosis pathways in MCAO models.