Study of Coleus Amboinicus Extract in Increasing of Transforming Growth Factor-BI (TGF-BI) Concentration and Docking Prediction Quer-cetin Derivatives in 4XOM Receptor on Uric Acid-Induced in Rats
Abstract: Background: Coleus arnboinicus extracts are known to have anti-oxidant activity, anti-platelet aggregations, anti bacteti al, anticancer and anti-inflammation. Objective: To evaluate Coleus amboi11icus (CA) extracts in increasing of transfonning growth factor-PI concentration and molecul...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article PeerReviewed |
| Language: | English English English |
| Published: |
Bentham Science Publishers B.V
2020
|
| Subjects: | |
| Online Access: | https://repository.unair.ac.id/107230/4/Study%20of%20Coleus%20Amboinicus%20Extract%20in%20Increasing.pdf https://repository.unair.ac.id/107230/7/Peer%20Review%20Study%20of%20Coleus%20Amboinicus%20Extract%20in%20Increasing.pdf https://repository.unair.ac.id/107230/5/Turnitin.pdf https://repository.unair.ac.id/107230/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Universitas Airlangga |
| Language: | English English English |
| Summary: | Abstract: Background: Coleus arnboinicus extracts are known to have anti-oxidant activity, anti-platelet aggregations, anti bacteti al, anticancer and anti-inflammation.
Objective: To evaluate Coleus amboi11icus (CA) extracts in increasing of transfonning growth factor-PI concentration and molecular docking prediction of quercetin on receptor 4X0M (TGF-p I) , measuring the levels of BUN, serum creatinine and Glutathione Peroxidase (GPx) on uric acid-induced rats.
Method: Fourty-two male Wistar rats (Rattus norvegicus), 3 months, 150-200 g were allocated into 3 groups (n=l 4). The control group received placebo (U-0), treatment group were administered orally with uric acid 1,5% and oxonic acid 2% (U- 1) and received 500 mg/kg bw of the CA extracts (U-2) respectively for 30 days. Blood serums collected for analysis of cre atinine and BUN concentrations. All groups were sacrificed to collect kidney for measuring of GPx activity and TGF-Pl concentration was conducted by avidin-horseradish peroxidase (HRP) sandwich-Elisa. Kidney organ was collected to histo pathological analyzed by HE and PAS staining.
Results: CA extract analyzed by TLC has a relative fraction of flavonoids, terpenes, saponins, polyphenols and alkaloids. Induction with uric acid has proven to causes acute renal failw·e with indicated of elevated BUN, serum creatinine concen tration and necrotic lesions of tubular membrane in treatment groups. Treatment of CA extract at a dose of 500 mg/kg bw could increase ofGPx and ofTGF-fll concenu·ation significantly (p-50.05). Quercetin as a marker compound of CA extract has stronger bind to the TGF-Plreceptor (PDB code: 4XOM) than its of 3WA_601 ligand by in sil ico.
Conclusion: CA extract is proven to inhibit acute renal failure by increasing of TGF-P lconcenu·ation and has strong binding of its receptor
Keywords: Coleus Amboinicus, Transforming Growth Factor-131 (TGF- 131) , Glut.athione Peroxidase (GPx), Quercetin, Uric Acid |
|---|