Blood Collections
IN MOUSE It is important to select the proper method of blood collection that corresponds to the volume required for your research purposes. Some methods are intended for survival and others are not. Retro-orbital Sinus Blood Collection The retro-orbital sinus is the site located behind the ey...
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| Format: | Conference or Workshop Item PeerReviewed |
| Language: | Indonesian English English |
| Published: |
2011
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| Online Access: | https://repository.unair.ac.id/107331/9/Prosiding%20Nasional%20Blood%20Collections_Rev.pdf https://repository.unair.ac.id/107331/10/Validasi.pdf https://repository.unair.ac.id/107331/6/Turnitin_compressed.pdf https://repository.unair.ac.id/107331/ |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian English English |
| Summary: | IN MOUSE
It is important to select the proper method of blood collection that corresponds to the volume required for your research purposes. Some methods are intended for survival and others are not. Retro-orbital Sinus Blood Collection
The retro-orbital sinus is the site located behind the eye at the medial or lateral canthus. This venous sinus is located just underneath the conjunctival membrane. This method is intended for survival blood collection. No more than 10% of the blood volume should be removed at one sampling. The blood volume of a mouse is approximately 8% of the body weight. For example, a 25 gram mouse has a blood volume of approximately 2 ml, so no more than 200 il of blood can be removed at a single bleeding without scientific justification and approval of the Animal Care and Use Committee. Mice should not be bled more frequently than every 3 weeks unless smaller volumes are collected.
Restrain the mouse by the scruff method. It is imperative that the mouse bet properly restrained. If the mouse is allowed to move its head, severe injury to the eye or surrounding tissues could occur. A topical ophthalmic anesthetic must be used prior to performing this procedure. Apply one drop of an anesthetic such as proparacaine or tetracaine hydrochloride to the eye. Be careful not to touch the tip of the applicator to any part of the mouse. This will cause contamination of the anesthetic. Wait 5-10 seconds
after the anesthetic is applied before attempting this procedure. Gently blot away excess anesthetic with a clean gauze pad, being careful not to scratch the cornea. An alternative to topical anesthesia for this procedure is general anesthesia. With a gentle rotating motion, insert the tube through the sinus membrane. Continue rotating the tube at the back of the orbit until blood flows. Collect the appropriate volume of blood. Upon completion, ensure good hemostasis with a clean gauze pad before returning the animal |
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