Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes
This study aimed to analyze the interaction of primaquine (PQ), chloroquine (CQ), and liposomes to support the design of optimal liposomal delivery for hepatic stage malaria infectious disease. The liposomes were composed of hydrogenated soybean phosphatidylcholine, cholesterol, and distearoyl-sn-gl...
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id-langga.1095082021-08-30T03:32:29Z http://repository.unair.ac.id/109508/ Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes Andang Miatmoko, - Ira Nurjannah, - Nuril Fadilatul Nehru, - Noorma Rosita, - Esti Hendradi, - Retno Sari, - Juni Ekowati, - R Medicine RS Pharmacy and materia medica RS1-441 Pharmacy and materia medica RS200-201 Pharmaceutical dosage forms This study aimed to analyze the interaction of primaquine (PQ), chloroquine (CQ), and liposomes to support the design of optimal liposomal delivery for hepatic stage malaria infectious disease. The liposomes were composed of hydrogenated soybean phosphatidylcholine, cholesterol, and distearoyl-sn-glycero-3-phosphoethanolamine-N-(methoxy[polyethyleneglycol]-2000), prepared by thin film method, then evaluated for physicochemical and spectrospic characteristics. The calcein release was further evaluated to determine the effect of drug co-loading on liposomal membrane integrity. The results showed that loading PQ and CQ into liposomes produced changes in the infrared spectra of the diester phosphate and carbonyl ester located in the polar part of the phospholipid, in addition to the alkyl group (CH2) in the nonpolar portion. Moreover, the thermogram revealed the loss of the endothermic peak of liposomes dually loaded with PQ and CQ at 186.6 °C, which is identical to that of the phospholipid. However, no crystallinity changes were detected through powder X-ray diffraction analysis. Moreover, PQ, with either single or dual loading, produced the higher calcein release profiles from the liposomes than that of CQ. The dual loading of PQ and CQ tends to interact with the polar head group of the phosphatidylcholine bilayer membrane resulted in an increase in water permeability of the liposomes. Nature Publishing Group 2021-06-14 Article PeerReviewed text en http://repository.unair.ac.id/109508/8/C-02%20Artikel.pdf text en http://repository.unair.ac.id/109508/7/C-02%20PEER%20REVIEW.pdf text en http://repository.unair.ac.id/109508/3/C-02%20SIMILARITY.pdf Andang Miatmoko, - and Ira Nurjannah, - and Nuril Fadilatul Nehru, - and Noorma Rosita, - and Esti Hendradi, - and Retno Sari, - and Juni Ekowati, - (2021) Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes. Nature, 11 (12420). pp. 1-12. ISSN 1476-4687 https://www.nature.com/articles/s41598-021-91866-0 https://doi.org/10.1038/s41598-021-91866-0 |
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R Medicine RS Pharmacy and materia medica RS1-441 Pharmacy and materia medica RS200-201 Pharmaceutical dosage forms Andang Miatmoko, - Ira Nurjannah, - Nuril Fadilatul Nehru, - Noorma Rosita, - Esti Hendradi, - Retno Sari, - Juni Ekowati, - Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes |
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This study aimed to analyze the interaction of primaquine (PQ), chloroquine (CQ), and liposomes to support the design of optimal liposomal delivery for hepatic stage malaria infectious disease. The liposomes were composed of hydrogenated soybean phosphatidylcholine, cholesterol, and distearoyl-sn-glycero-3-phosphoethanolamine-N-(methoxy[polyethyleneglycol]-2000), prepared by thin film method, then evaluated for physicochemical and spectrospic characteristics. The calcein release was further evaluated to determine the effect of drug co-loading on liposomal membrane integrity. The results showed that loading PQ and CQ into liposomes produced changes in the infrared spectra of the diester phosphate and carbonyl ester located in the polar part of the phospholipid, in addition to the alkyl group (CH2) in the nonpolar portion. Moreover, the thermogram revealed the loss of the endothermic peak of liposomes dually loaded with PQ and CQ at 186.6 °C, which is identical to that of the phospholipid. However, no crystallinity changes were detected through powder X-ray diffraction analysis. Moreover, PQ, with either single or dual loading, produced the higher calcein release profiles from the liposomes than that of CQ. The dual loading of PQ and CQ tends to interact with the polar head group of the phosphatidylcholine bilayer membrane resulted in an increase in water permeability of the liposomes. |
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Article PeerReviewed |
author |
Andang Miatmoko, - Ira Nurjannah, - Nuril Fadilatul Nehru, - Noorma Rosita, - Esti Hendradi, - Retno Sari, - Juni Ekowati, - |
author_facet |
Andang Miatmoko, - Ira Nurjannah, - Nuril Fadilatul Nehru, - Noorma Rosita, - Esti Hendradi, - Retno Sari, - Juni Ekowati, - |
author_sort |
Andang Miatmoko, - |
title |
Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes |
title_short |
Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes |
title_full |
Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes |
title_fullStr |
Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes |
title_full_unstemmed |
Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes |
title_sort |
interactions of primaquine and chloroquine with pegylated phosphatidylcholine liposomes |
publisher |
Nature Publishing Group |
publishDate |
2021 |
url |
http://repository.unair.ac.id/109508/8/C-02%20Artikel.pdf http://repository.unair.ac.id/109508/7/C-02%20PEER%20REVIEW.pdf http://repository.unair.ac.id/109508/3/C-02%20SIMILARITY.pdf http://repository.unair.ac.id/109508/ https://www.nature.com/articles/s41598-021-91866-0 https://doi.org/10.1038/s41598-021-91866-0 |
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