Antigenic Site Of Glycoprotein Encoding Gene In Rabies Virus Isolate From Indonesia
Rabies is one of the most harmful zoonotic diseases in the world, affecting both animal and human health. Each year, 55,000 people die from rabies: 56% of deaths are in Asia and 54% in Africa. In Indonesia, rabies cases (98%) are usually transmitted by dogs (domestic and wild dogs) (96.79%), cats (1...
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| Main Authors: | , , |
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| Format: | Article PeerReviewed |
| Language: | English English English |
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The College of Veterinary Medicine University of the Philippines Los Banos
2018
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| Subjects: | |
| Online Access: | https://repository.unair.ac.id/111195/8/C3%20Philippine%20Journal%20of%20Veterinary%20Medicine.pdf https://repository.unair.ac.id/111195/2/Validasi%20dan%20Peer%20Review.pdf https://repository.unair.ac.id/111195/9/Turnitin.pdf https://repository.unair.ac.id/111195/ https://ejournals.ph/article.php?id=13523 |
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| Institution: | Universitas Airlangga |
| Language: | English English English |
| Summary: | Rabies is one of the most harmful zoonotic diseases in the world, affecting both animal and human health. Each year, 55,000 people die from rabies: 56% of deaths are in Asia and 54% in Africa. In Indonesia, rabies cases (98%) are usually transmitted by dogs (domestic and wild dogs) (96.79%), cats (1.06%) and apes (0.15%). The aim of this study was to determine the homology score and predict the antigenic sites of the glycoprotein (G-protein) encoding gene from rabies virus in Indonesia using molecular analyses. G-protein gene from isolated samples was amplified using a two-step RT-PCR followed by sequencing. Results show that homology scores of Indonesian rabies virus isolates against reference virus isolates obtained from GenBank records are the following: Indonesia (93-98%), China (89-91%), Thailand (83-86%), India (82-84%), Korea (83-85%) and Pasteur strain (82-83%). Only antigenic site I of isolated sample from Sumatra changed, while antigenic sites II and VI remain unchanged. This suggests that another G-gene strain from a different region might exist in rabies virus isolated from Indonesia.
Keywords : amino acid, antigenic site, dog, G-gene, Indonesia, rabies virus |
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