Chondrogenic Differentiation Capacity of Human Umbilical Cord Mesenchymal Stem Cells with Platelet Rich Fibrin Scaffold in Cartilage Regeneration (In Vitro Study)

• Main Authors: Ni Putu Mira Sumarta, -, Coen Pramono Danudiningrat, -, Eryk Hendrianto, -, Helen Susilowati, -, Deya Karsari, -, Fedik A. Rantam, -
• Format: Article PeerReviewed
• Language: English; Indonesian; English; English; English
• Published: Cross Mark 2016
• Subjects: R Medicine; RK Dentistry
• Online Access: https://repository.unair.ac.id/113487/1/1%20Chondrogenic%20Differentiation%20Capacity.pdf; https://repository.unair.ac.id/113487/3/1_%20Chondrogenic%20Differentiation%20Capacity%20of%20Human%20Umbilical%20Cord%20Mesenchymal%20Stem%20Cells%20with%20Platelet%20Rich%20Fibrin%20Scaffold%20in%20Cartilage%20Regeneration%20%28In%20Vitro%20Study%29.pdf; https://repository.unair.ac.id/113487/6/1.pdf; https://repository.unair.ac.id/113487/8/Chondrogenic%20Differentiation%20Capacity%20of%20Human%20Umbilical%20Cord%20Mesenchymal%20Stem%20Cells.pdf; https://repository.unair.ac.id/113487/10/koresponden%20Chondrogenic.pdf; https://repository.unair.ac.id/113487/
• Institution: Universitas Airlangga

Human umbilical cord mesenchymal stem cell is a promising source of allogenous MSC with great chondrogenic differentiation capacity. Meanwhile, platelet rich fibrin (PRF) is a natural fibrin matrix, rich in growth factors, forming a smooth and flexible fibrin network, supporting cytokines and cell migration, thus can be used as a scaffold that facilitate the differentiation of MSC. However, the differential capability of MSC cultured in PRF was still poorly understood. Method: We studied in vitro differentiation potential of MSC cultured in PRF by evaluating several markers such as FGF 18, Sox 9, type II collagen, aggrecan in 3 different culture medium. Result: The result showed that there was positive expression of FGF 18, Sox 9, type II collagen, aggrecan in all medium of in vitro culture. Conclusion: MSC cultured from human umbilical cord had the capacity of chondrogenic differentiation and able to produce cartilage extracellular matrix in vitro which means that hUCMSC is a potential allogeneic MSC for cartilage regeneration.