Moleculer Detection of Entamoeba spp in Long-tailed Macaque (Macaca fascicularis) at Baluran National Park, Indonesia
ntroduction: : Baluran National Park (BNP) is one of the oldest national parks in Indonesia. Due to its natural beauty, BNP is also the highest number of tourists in Indonesia. Macaca fascicularis is one of the species that has a high population abundance in several park locations. Several species o...
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2021
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id-langga.1137682022-02-23T03:57:48Z https://repository.unair.ac.id/113768/ Moleculer Detection of Entamoeba spp in Long-tailed Macaque (Macaca fascicularis) at Baluran National Park, Indonesia Nunuk Dyah Retno Lastuti Lucia Tri Suwanti Poedji Hastutiek Dyah Ayu Kurniawati Heni Puspitasari SF408-408.6 Zoo animals SF811-909 Veterinary medicine of special organs, regions, and systems ntroduction: : Baluran National Park (BNP) is one of the oldest national parks in Indonesia. Due to its natural beauty, BNP is also the highest number of tourists in Indonesia. Macaca fascicularis is one of the species that has a high population abundance in several park locations. Several species of Entamoeba have been reported as pathogenic and non-pathogenic to humans and Non-human primates (NHP). The aim of this research was to detect gen 18 SSU rDNA of Entamoeba spp in long-tailed macaque at Baluran National Park. Methods: A total 100 faecal samples of Macaca fascicularis taken from BNP, Indonesia. Samples were purified with sugar flotation methods, and extracted the DNA using DNAzol. For molecular identification of Entamoeba spp, using primer pairs Entamoeba-specific primers, including Entam1 (5-GTT GAT CCT GCC AGT ATT ATA TG-3) and Entam2 (5-CAC TAT TGG AGC TGG AAT TAC-3), have been designed by Verweij et al. Amplification is carried out 5 min at 95° C; 40 denaturation cycles of 30 s, 95° C; 30 s for annealing at 57° C, and 30 s for extension at 72° C, with a final step of 2 min at 72°C. Results: The prevalence of Entamoeba spp. based on PCR examination was 53% (53/100). The PCR products from Entamoeba spp. isolates from Macaca fascicularis produced bands of around 600 bp with 2% agarose gel electrophoresis. Conclusion: there was a high prevalence of Entamoeba spp in Macaca fascicularis in BNP, and E. coli were identified with 18 SSU rDNA genes. Keywords Baluran National ParkEntamoeba spp.IndonesiaMacaca fascicularis Universiti Putra Malaysia Press 2021-04 Article PeerReviewed text en https://repository.unair.ac.id/113768/3/Bukti%20Moleculer%20Detection.pdf text en https://repository.unair.ac.id/113768/4/Peer%20Review%20Moleculer%20Detection.pdf text en https://repository.unair.ac.id/113768/1/Turn%20It%20In%20Molecular%20Detection.pdf Nunuk Dyah Retno Lastuti and Lucia Tri Suwanti and Poedji Hastutiek and Dyah Ayu Kurniawati and Heni Puspitasari (2021) Moleculer Detection of Entamoeba spp in Long-tailed Macaque (Macaca fascicularis) at Baluran National Park, Indonesia. Malaysian Journal of Medicine and Helath Sciences, 17 (SUPP2). pp. 85-88. ISSN 2636-9346 |
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SF408-408.6 Zoo animals SF811-909 Veterinary medicine of special organs, regions, and systems Nunuk Dyah Retno Lastuti Lucia Tri Suwanti Poedji Hastutiek Dyah Ayu Kurniawati Heni Puspitasari Moleculer Detection of Entamoeba spp in Long-tailed Macaque (Macaca fascicularis) at Baluran National Park, Indonesia |
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ntroduction: : Baluran National Park (BNP) is one of the oldest national parks in Indonesia. Due to its natural beauty, BNP is also the highest number of tourists in Indonesia. Macaca fascicularis is one of the species that has a high population abundance in several park locations. Several species of Entamoeba have been reported as pathogenic and non-pathogenic to humans and Non-human primates (NHP). The aim of this research was to detect gen 18 SSU rDNA of Entamoeba spp in long-tailed macaque at Baluran National Park. Methods: A total 100 faecal samples of Macaca fascicularis taken from BNP, Indonesia. Samples were purified with sugar flotation methods, and extracted the DNA using DNAzol. For molecular identification of Entamoeba spp, using primer pairs Entamoeba-specific primers, including Entam1 (5-GTT GAT CCT GCC AGT ATT ATA TG-3) and Entam2 (5-CAC TAT TGG AGC TGG AAT TAC-3), have been designed by Verweij et al. Amplification is carried out 5 min at 95° C; 40 denaturation cycles of 30 s, 95° C; 30 s for annealing at 57° C, and 30 s for extension at 72° C, with a final step of 2 min at 72°C. Results: The prevalence of Entamoeba spp. based on PCR examination was 53% (53/100). The PCR products from Entamoeba spp. isolates from Macaca fascicularis produced bands of around 600 bp with 2% agarose gel electrophoresis. Conclusion: there was a high prevalence of Entamoeba spp in Macaca fascicularis in BNP, and E. coli were identified with 18 SSU rDNA genes.
Keywords Baluran National ParkEntamoeba spp.IndonesiaMacaca fascicularis |
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Article PeerReviewed |
author |
Nunuk Dyah Retno Lastuti Lucia Tri Suwanti Poedji Hastutiek Dyah Ayu Kurniawati Heni Puspitasari |
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Nunuk Dyah Retno Lastuti Lucia Tri Suwanti Poedji Hastutiek Dyah Ayu Kurniawati Heni Puspitasari |
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Nunuk Dyah Retno Lastuti |
title |
Moleculer Detection of Entamoeba spp in Long-tailed Macaque (Macaca fascicularis) at Baluran National Park, Indonesia |
title_short |
Moleculer Detection of Entamoeba spp in Long-tailed Macaque (Macaca fascicularis) at Baluran National Park, Indonesia |
title_full |
Moleculer Detection of Entamoeba spp in Long-tailed Macaque (Macaca fascicularis) at Baluran National Park, Indonesia |
title_fullStr |
Moleculer Detection of Entamoeba spp in Long-tailed Macaque (Macaca fascicularis) at Baluran National Park, Indonesia |
title_full_unstemmed |
Moleculer Detection of Entamoeba spp in Long-tailed Macaque (Macaca fascicularis) at Baluran National Park, Indonesia |
title_sort |
moleculer detection of entamoeba spp in long-tailed macaque (macaca fascicularis) at baluran national park, indonesia |
publisher |
Universiti Putra Malaysia Press |
publishDate |
2021 |
url |
https://repository.unair.ac.id/113768/3/Bukti%20Moleculer%20Detection.pdf https://repository.unair.ac.id/113768/4/Peer%20Review%20Moleculer%20Detection.pdf https://repository.unair.ac.id/113768/1/Turn%20It%20In%20Molecular%20Detection.pdf https://repository.unair.ac.id/113768/ |
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