Precipitation Solvents For Rna Extraction Of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, And Ultrapure H2o
Dengue is a disease caused by a virus from the family Flaviviradae, carried by a female mosquito of Aedes aegypti species. Dengue fever is widespread in the tropic areas. It caused by rainfall, temperature and unplanned urbanization. According to the ministry of health , almost all provinces in Indo...
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Departemen Kimia Fakultas Sains dan Teknologi Universitas Airlangga
2018
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id-langga.1138352022-03-01T03:15:57Z https://repository.unair.ac.id/113835/ Precipitation Solvents For Rna Extraction Of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, And Ultrapure H2o Rizqidhana Juliana Putri, - Teguh Hari Sucipto, - Harsasi Setyawati, - Siti Churrotin, - Ilham Harlan Amarullah, - Puspa Wardhani, - Aryati, - Soegeng Soegijanto, - R Medicine (General) RB37-56.5 Clinical pathology. Laboratory technique Dengue is a disease caused by a virus from the family Flaviviradae, carried by a female mosquito of Aedes aegypti species. Dengue fever is widespread in the tropic areas. It caused by rainfall, temperature and unplanned urbanization. According to the ministry of health , almost all provinces in Indonesia are endemic areas of dengue fever. In 2014, up to mid-December Dengue Hemorrhagic Fever (DHF) patients in 34 provinces in Indonesia are 71,668 people and 641. This figure is lower than the previous year, 2013 with 112,511 people and 871 deaths . This disease consists of four types of serotypes, namely DENV-1, DENV-2, DENV-3, and DENV-4. This disease can be identified using a variety of methods, one of the method is Reverse Transcription - Polymerase Chain Reaction (RT-PCR) method. This study aims to determine the ability of Dimethylformamide (DMF), Ethylenediamintetraacetic Acid (EDTA), and Ultrapure H2O as the substitute of Ethanol for precipitation in RNA extraction process. The sample used in this research obtained from Surabaya. RNA extraction itself can be done by using a special kit for RNA extraction. In Reverse Transcription - Polymerase Chain Reaction method, first RNA is extracted and then transcribed back (Reverse Transcription) which then form cDNA that later will be amplified by using PCR method. In this study used specific primers for dengue virus type 3 (DENV-3). The results of this study show that DMF, EDTA, and Ultrapure H2O can be used as the substitute of Ethanol for precipitation on RNA extraction. The result is evidenced by the formation of viral DNA bands on gel electrophoresis results. Departemen Kimia Fakultas Sains dan Teknologi Universitas Airlangga 2018 Article PeerReviewed text en https://repository.unair.ac.id/113835/1/33%20Artikel.pdf text en https://repository.unair.ac.id/113835/2/33%20Similarity.pdf text en https://repository.unair.ac.id/113835/3/33.pdf Rizqidhana Juliana Putri, - and Teguh Hari Sucipto, - and Harsasi Setyawati, - and Siti Churrotin, - and Ilham Harlan Amarullah, - and Puspa Wardhani, - and Aryati, - and Soegeng Soegijanto, - (2018) Precipitation Solvents For Rna Extraction Of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, And Ultrapure H2o. Jurnal Kimia Riset, 3 (2). pp. 78-83. ISSN 2528-0422 https://e-journal.unair.ac.id/JKR/article/view/9353 http://dx.doi.org/10.20473/jkr.v3i2.9353 |
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R Medicine (General) RB37-56.5 Clinical pathology. Laboratory technique Rizqidhana Juliana Putri, - Teguh Hari Sucipto, - Harsasi Setyawati, - Siti Churrotin, - Ilham Harlan Amarullah, - Puspa Wardhani, - Aryati, - Soegeng Soegijanto, - Precipitation Solvents For Rna Extraction Of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, And Ultrapure H2o |
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Dengue is a disease caused by a virus from the family Flaviviradae, carried by a female mosquito of Aedes aegypti species. Dengue fever is widespread in the tropic areas. It caused by rainfall, temperature and unplanned urbanization. According to the ministry of health , almost all provinces in Indonesia are endemic areas of dengue fever. In 2014, up to mid-December Dengue Hemorrhagic Fever (DHF) patients in 34 provinces in Indonesia are 71,668 people and 641. This figure is lower than the previous year, 2013 with 112,511 people and 871 deaths . This disease consists of four types of serotypes, namely DENV-1, DENV-2, DENV-3, and DENV-4. This disease can be identified using a variety of methods, one of the method is Reverse Transcription - Polymerase Chain Reaction (RT-PCR) method. This study aims to determine the ability of Dimethylformamide (DMF), Ethylenediamintetraacetic Acid (EDTA), and Ultrapure H2O as the substitute of Ethanol for precipitation in RNA extraction process. The sample used in this research obtained from Surabaya. RNA extraction itself can be done by using a special kit for RNA extraction. In Reverse Transcription - Polymerase Chain Reaction method, first RNA is extracted and then transcribed back (Reverse Transcription) which then form cDNA that later will be amplified by using PCR method. In this study used specific primers for dengue virus type 3 (DENV-3). The results of this study show that DMF, EDTA, and Ultrapure H2O can be used as the substitute of Ethanol for precipitation on RNA extraction. The result is evidenced by the formation of viral DNA bands on gel electrophoresis results. |
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Rizqidhana Juliana Putri, - Teguh Hari Sucipto, - Harsasi Setyawati, - Siti Churrotin, - Ilham Harlan Amarullah, - Puspa Wardhani, - Aryati, - Soegeng Soegijanto, - |
author_facet |
Rizqidhana Juliana Putri, - Teguh Hari Sucipto, - Harsasi Setyawati, - Siti Churrotin, - Ilham Harlan Amarullah, - Puspa Wardhani, - Aryati, - Soegeng Soegijanto, - |
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Rizqidhana Juliana Putri, - |
title |
Precipitation Solvents For Rna Extraction Of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, And Ultrapure H2o |
title_short |
Precipitation Solvents For Rna Extraction Of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, And Ultrapure H2o |
title_full |
Precipitation Solvents For Rna Extraction Of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, And Ultrapure H2o |
title_fullStr |
Precipitation Solvents For Rna Extraction Of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, And Ultrapure H2o |
title_full_unstemmed |
Precipitation Solvents For Rna Extraction Of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, And Ultrapure H2o |
title_sort |
precipitation solvents for rna extraction of dengue virus type 3: dimethylformamide, ethylenediamintetraacetic acid, and ultrapure h2o |
publisher |
Departemen Kimia Fakultas Sains dan Teknologi Universitas Airlangga |
publishDate |
2018 |
url |
https://repository.unair.ac.id/113835/1/33%20Artikel.pdf https://repository.unair.ac.id/113835/2/33%20Similarity.pdf https://repository.unair.ac.id/113835/3/33.pdf https://repository.unair.ac.id/113835/ https://e-journal.unair.ac.id/JKR/article/view/9353 http://dx.doi.org/10.20473/jkr.v3i2.9353 |
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1726147216099246080 |