Profil Subkelas IgG Serum Mencit Anti-Toxocara Canis Sebagai Asar Pemilihan Marker Spesifik Untuk Pemeriksaan Antibodi Pada Kasus Toxocariasis
The objective of this study was to get the specific protein of T. canis and to in estigate the dominant immunoglobulin that could be used as a biomarker in kit di gnostic to toxocariasis diagnosis through antibody test by indirect-ELISA technique. This study was done to isolate and characterize T....
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| Format: | Monograph NonPeerReviewed |
| Language: | Indonesian |
| Published: |
UNIVERSITAS AIRLANGGA
2009
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| Online Access: | https://repository.unair.ac.id/115805/1/2022_03_28_11_05_15.pdf https://repository.unair.ac.id/115805/ http://www.lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian |
| Summary: | The objective of this study was to get the specific protein of T. canis and to in estigate the dominant immunoglobulin that could be used as a biomarker in kit di gnostic to toxocariasis diagnosis through antibody test by indirect-ELISA technique.
This study was done to isolate and characterize T. canis protein from e cretory-excretory (ES) material of 12 dormant by pure protein that was reactive with p Iyclonal antibody. !n the first step, 12 dormant were homogenized by sonication 3 30 seconds and identified by SOS-PAGE with silver staining. The second step, p tein was transferred to nitrocellulose membrane used polyclonal antibody of antidormant that was visualized through goat-anti rabbit conjugate and SCIP+NST st ining. The third step was decided the specific of protein fractions that was done b se on molecule weight (MW) and the specific protein was isolated by elution te hnique. The fourth was to evaluate the antigenicity, sensitivity and specificity of c de and pure protein by IgG-ELISA as response against T. canis infection toward m e and to study the profile IgG and the dominant of subclass IgG as response ag inst T. canis infection toward mice.
The results of [he study that: 1) T. canis have more protein with variation of m lecular weight between 8 and 200 kOa; it also the specific and cross, reaction pr teins of T. cati and antibody against T. canis; 2) The specific proteins of T. cati that and recognized by the anti-To canis sera are as follow proteins of MW 168,120,80 and 70 kOa; 3) The specific subclass of Ig G in the anti-To canis mice sera were IgG2a; 4) Saed on the ELISA 00 values, pure protein Toxocara spp. have high antigenicity of se um anti-Toxocara spp., they have a lower antigenicity against other worms (A cylostoma spp. and D. ,caninum), with a value of 1000.4 sensitivity and specificity of 875%. |
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