Identifikasi MDR (Multi Drugs Resistance) melalui Gen Intl (Lntegron Class 1) Citrobacter Freundii pada Telur Ayam dengan Metode PCR (Polymerase Chain Reactfon)
Chicken eggs are a rich source of protein; therefore, eggs are easily contaminated by bacteria, especially bacteria from the Enterobacteriaceae family. C. freundii is a member of the Enterobacteriaceae family that can cause gastrointestinal infections in humans through the consumption of contamin...
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id-langga.1190822022-12-06T04:22:12Z https://repository.unair.ac.id/119082/ Identifikasi MDR (Multi Drugs Resistance) melalui Gen Intl (Lntegron Class 1) Citrobacter Freundii pada Telur Ayam dengan Metode PCR (Polymerase Chain Reactfon) Nindita Lestyana Putri SF481-507 Poultry. Eggs Chicken eggs are a rich source of protein; therefore, eggs are easily contaminated by bacteria, especially bacteria from the Enterobacteriaceae family. C. freundii is a member of the Enterobacteriaceae family that can cause gastrointestinal infections in humans through the consumption of contaminated food. Besides being fOlmd on food, C. freundii is also often isolated from soil, water, feces, waste materials, and intestines. C. freundii plays an active role in carrying a resistance gene in the gastrointestinal tract of both humans and animals and can contaminate animalbased food products through feces. This study aimed to discover whether the MDRcoding gene, Inti, could be found in C. freundii isolates. The isolation and identification of C. freundii in this study used LB as the pre-enrichment medium, TTB as the enrichment medium, and the medium SSA as a selective medium, followed by Gram staining of colonies, biochemical tests (TSIA, Urea, SeA, SIM, MR-VP), carbohydrate fermentation tests (lactose, sucrose, glucose, mannitol, and maltose) and a validation test using the Vitek 2 machine. Tests for resistance to a number of antibiotics using the antibiotic disc diffusion method were conducted for streptomycin, gentamycin, chloramphenicol, erythromycin, tetracycline, oxytetracycline, doxycycline, amoxicillin, penicillin, oxacillin, cloxacillin, trimethoprim, and ciprofloxacin. The results study demonstrated that C. freundii was 100% resistant to erythromycin, amoxicillin, penicillin, oxacillin, and cloxacillin, and 33.3% resistant to ampicillin. The Polymerase Chain Reaction (PCR) analysis of C. freundii revealed DNA strand fragments at a fragment length of 346 bp. The PCR product sequencing analysis was conducted to see the homology of the DNA. The result of the homology was 91% by using the BLAST system. 2017-01-30 Thesis NonPeerReviewed text en https://repository.unair.ac.id/119082/1/KKC%20KK%20TPKMV%2008_19%20Put%20i_ABSTRAK.pdf text en https://repository.unair.ac.id/119082/2/KKC%20KK%20TPKMV%2008_19%20Put%20i.pdf Nindita Lestyana Putri (2017) Identifikasi MDR (Multi Drugs Resistance) melalui Gen Intl (Lntegron Class 1) Citrobacter Freundii pada Telur Ayam dengan Metode PCR (Polymerase Chain Reactfon). Thesis thesis, UNIVERSITAS AIRLANGGA. http://lib.unair.ac.id |
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SF481-507 Poultry. Eggs Nindita Lestyana Putri Identifikasi MDR (Multi Drugs Resistance) melalui Gen Intl (Lntegron Class 1) Citrobacter Freundii pada Telur Ayam dengan Metode PCR (Polymerase Chain Reactfon) |
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Chicken eggs are a rich source of protein; therefore, eggs are easily
contaminated by bacteria, especially bacteria from the Enterobacteriaceae family. C.
freundii is a member of the Enterobacteriaceae family that can cause gastrointestinal
infections in humans through the consumption of contaminated food. Besides being
fOlmd on food, C. freundii is also often isolated from soil, water, feces, waste
materials, and intestines. C. freundii plays an active role in carrying a resistance gene
in the gastrointestinal tract of both humans and animals and can contaminate animalbased
food products through feces. This study aimed to discover whether the MDRcoding
gene, Inti, could be found in C. freundii isolates. The isolation and
identification of C. freundii in this study used LB as the pre-enrichment medium,
TTB as the enrichment medium, and the medium SSA as a selective medium,
followed by Gram staining of colonies, biochemical tests (TSIA, Urea, SeA, SIM,
MR-VP), carbohydrate fermentation tests (lactose, sucrose, glucose, mannitol, and
maltose) and a validation test using the Vitek 2 machine. Tests for resistance to a
number of antibiotics using the antibiotic disc diffusion method were conducted for
streptomycin, gentamycin, chloramphenicol, erythromycin, tetracycline,
oxytetracycline, doxycycline, amoxicillin, penicillin, oxacillin, cloxacillin,
trimethoprim, and ciprofloxacin. The results study demonstrated that C. freundii was
100% resistant to erythromycin, amoxicillin, penicillin, oxacillin, and cloxacillin, and
33.3% resistant to ampicillin. The Polymerase Chain Reaction (PCR) analysis of C.
freundii revealed DNA strand fragments at a fragment length of 346 bp. The PCR
product sequencing analysis was conducted to see the homology of the DNA. The
result of the homology was 91% by using the BLAST system. |
format |
Theses and Dissertations NonPeerReviewed |
author |
Nindita Lestyana Putri |
author_facet |
Nindita Lestyana Putri |
author_sort |
Nindita Lestyana Putri |
title |
Identifikasi MDR (Multi Drugs Resistance) melalui Gen
Intl (Lntegron Class 1) Citrobacter Freundii pada Telur Ayam
dengan Metode PCR (Polymerase Chain Reactfon) |
title_short |
Identifikasi MDR (Multi Drugs Resistance) melalui Gen
Intl (Lntegron Class 1) Citrobacter Freundii pada Telur Ayam
dengan Metode PCR (Polymerase Chain Reactfon) |
title_full |
Identifikasi MDR (Multi Drugs Resistance) melalui Gen
Intl (Lntegron Class 1) Citrobacter Freundii pada Telur Ayam
dengan Metode PCR (Polymerase Chain Reactfon) |
title_fullStr |
Identifikasi MDR (Multi Drugs Resistance) melalui Gen
Intl (Lntegron Class 1) Citrobacter Freundii pada Telur Ayam
dengan Metode PCR (Polymerase Chain Reactfon) |
title_full_unstemmed |
Identifikasi MDR (Multi Drugs Resistance) melalui Gen
Intl (Lntegron Class 1) Citrobacter Freundii pada Telur Ayam
dengan Metode PCR (Polymerase Chain Reactfon) |
title_sort |
identifikasi mdr (multi drugs resistance) melalui gen
intl (lntegron class 1) citrobacter freundii pada telur ayam
dengan metode pcr (polymerase chain reactfon) |
publishDate |
2017 |
url |
https://repository.unair.ac.id/119082/1/KKC%20KK%20TPKMV%2008_19%20Put%20i_ABSTRAK.pdf https://repository.unair.ac.id/119082/2/KKC%20KK%20TPKMV%2008_19%20Put%20i.pdf https://repository.unair.ac.id/119082/ http://lib.unair.ac.id |
_version_ |
1751554096119152640 |