Mesenchymal Stem Cells Transplantation in Endometriosis Model Mice on VEGF mRNA Expression to Improve Infertility

• Main Authors: Sri Ratna Dwiningsih, -, Hendy Hendarto, -, Fedik Abdul Rantam, -, Erry Gumilar Dachlan, -, I.W.A. Wiyasa, -, Widjiati, -
• Format: Article PeerReviewed
• Language: English; English; English; English
• Published: Ektodermal Displazi Grubu 2021
• Subjects: R Medicine (General); RG Gynecology and obstetrics
• Online Access: https://repository.unair.ac.id/119315/3/Similarity%207.pdf; https://repository.unair.ac.id/119315/7/7.pdf; https://repository.unair.ac.id/119315/9/Artikel%207.pdf; https://repository.unair.ac.id/119315/11/Bukti%20Korespondensi%207.pdf; https://repository.unair.ac.id/119315/; http://www.jidmr.com/journal/wp-content/uploads/2021/10/53-M21_1500_Widjiati_Indonesia.pdf
• Institution: Universitas Airlangga

The concentration of pro-inflammatory interleukin increases in the follicular fluid of patients with endometriosis, causing a decrease in the concentration of VEGF in this follicular fluid, which is thought to cause a decrease in embryo quality and a decrease in implantation rates. This study aimed to determine the role of VEGF mRNA in endometriosis repair. The study consisted of 3 groups, the negative control group (T0), the endometriosis model group that received placebo (T1), and received bone marrow mesenchymal stem cells (T2). Examination of VEGF mRNA expression using real-time PCR method. Data analysis using ANOVA statistical test. The results showed that the expression of VEGF mRNA between the endometriosis model group was significantly different from the endometriosis model group that received bone marrow mesenchymal stem cells, but it was not different from the control group or normal mice. This study concluded that the expression of VEGF mRNA in the group of endometriosis model mice that received bone marrow mesenchymal stem cells was lower than the group that did not. There was no relationship between VEGF mRNA expression and the number of primary follicles, secondary follicles, tertiary follicles, and de Graafian follicles.