The efficacy of topical human amniotic membrane-mesenchymal stem cell-conditioned medium (hAMMSC-CM) and a mixture of topical hAMMSC-CM + vitamin C and hAMMSC-CM + vitamin E on chronic plantar ulcers in leprosy:a randomized control trial

• Main Authors: Cita Rosita Sigit Prakoeswa, -, F. R. Natallya, -, D. Harnindya, -, A. Thohiroh, -, R. N. Oktaviyanti, -, K. D. Pratiwi, -, M. A. Rubianti, -, B. Yogatri, -, P. I. Primasari, -, N. Herwanto, -, Medhi Denisa Alinda, -, B. H. Kusumaputra, -, Linda Astari, -, M. Yulianto Listiawan, -, Indropo Agusni, -, Fedik Abdul Rantam, -
• Format: Article PeerReviewed
• Language: English; English; English
• Published: Informa Healthcare 2018
• Subjects: R Medicine (General); RL Dermatology
• Online Access: https://repository.unair.ac.id/119587/1/Artikel.pdf; https://repository.unair.ac.id/119587/2/Karil.pdf; https://repository.unair.ac.id/119587/3/Similarity.pdf; https://repository.unair.ac.id/119587/; https://www.tandfonline.com/doi/abs/10.1080/09546634.2018.1467541?journalCode=ijdt20; https://doi.org/10.1080/09546634.2018.1467541
• Institution: Universitas Airlangga

Background: Healing of chronic plantar ulcers in leprosy (CPUL) typically takes a long time due to impaired neurological function, thereby reducing the levels of growth factors and cytokines. Cytokines can be found in metabolite products from amniotic membrane stem cells. Chronic ulcers are frequently characterized by high levels of reactive oxygen species. Vitamin E (α-tocopherol) is widely used in skin lesions, owing to its antioxidant and anti-inflammatory properties. Vitamin C also has antioxidant, anti-inflammatory, and collagen synthesis properties which are useful in wound healing. Herein, we compared the effects of topical human amniotic membrane-mesenchymal stem cell-conditioned medium (hAMMSC-CM) alone and with vitamins C and E on healing of CPUL. Methods: In this randomized controlled trial, topical agents were applied every 3 days for up to 8 weeks. Ulcer size, side-effects, and possible complications were monitored weekly. Results: Healing percentage increased each week in all groups. Mean difference in ulcer size was highest in the hAMMSC-CM + vitamin E group, implying better progress of wound healing. There were no side-effects or complications. Conclusions: hAMMSC-CM + vitamin E is best for healing of CPUL.