Identifikasi Protein Hemaglutinin Virus AI Subtipe H5N1 Sebagai Kandidat Antigen Diagnostik
The objectives of this study were to find the molecular weight of hemaglutinin protein and its antigenicity if being reacted with anti-H5Nl , anti-H5N2 and antiH5N9 antibodies.The first step conducted in this study was to characterize hemaglutinin protein of AI virus H5Nl subtype using SDS-PAGE....
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian |
| Published: |
2008
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| Subjects: | |
| Online Access: | https://repository.unair.ac.id/121124/1/KK%20KKA%20TI%2007-11%20Yun%20i.pdf https://repository.unair.ac.id/121124/ http://www.lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian |
| Summary: | The objectives of this study were to find the molecular weight of hemaglutinin
protein and its antigenicity if being reacted with anti-H5Nl , anti-H5N2 and antiH5N9
antibodies.The first step conducted in this study was to characterize hemaglutinin protein
of AI virus H5Nl subtype using SDS-PAGE. Subsequently, to identify the
antigenicity of the protein against anti-H5Nl we conducted Western Blotting test and,
finally, we used indirect ELISA to find the antigenicity between hemaglutinin protein
and anti-H5Nl, anti-H5N2 and anti-H5N9 antibodies.The protein bands of HA, NP, NA, NS and Ml with molecular weights of 78 kDa; 59,4 kDa; 53,5 kDa; 27,9 kDa and 16,9 kDa were apparent in the gel as the result of SDS-PAGE. Using Western Blotting technique, the antigenicity of
hemaglutinin (HA) protein has been proved after being reacted with anti-H5N 1
antibody. Finally, indirect ELISA technique was used to test antigenicity between HA
protein and anti-H5N 1, anti H5N2 and anti H5N9 antibodies. The result of
examination, which was stated in Optical Density (OD), revealed ranges as follows:
0.3280-0,4120; 0.1150-0,2870 and 0.1360-0,3250. From the result of HSD, it was
found that anti-H5N1 antibody had the strongest interaction with hemaglutinin
protein.Based on this study, it can be concluded that hemaglutinin protein of AI virus
H5Nl subtype is antigenic against anti-H5Nl, anti-H5N2 and anti-H5N9 antibodies,
so that it can be used as candidate for diagnostic antigene. |
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