Exfoliated Human Deciduous Tooth Stem Cells Incorporating Carbonate Apatite Scaffold Enhance BMP-2, BMP-7 and Attenuate MMP-8 Expression During Initial Alveolar Bone Remodeling in Wistar Rats (Rattus norvegicus)
Background: Post-tooth extraction socket preservation is necessary due to alveolar bone resorptive patterns through regenerative dentistry approaches that involve the use of stem cells, scaffold and growth factor. Stem cells derived from human exfoliated deciduous teeth (SHED) are known to potent...
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Main Authors: | , , , , , |
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Format: | Article PeerReviewed |
Language: | English English English English |
Published: |
Dove Medical Press Ltd.
2020
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Subjects: | |
Online Access: | https://repository.unair.ac.id/122719/1/6..pdf https://repository.unair.ac.id/122719/2/6..pdf https://repository.unair.ac.id/122719/3/6..pdf https://repository.unair.ac.id/122719/4/6.Exfoliated.pdf https://repository.unair.ac.id/122719/ https://www.dovepress.com/getfile.php?fileID=56984 |
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Institution: | Universitas Airlangga |
Language: | English English English English |
Summary: | Background: Post-tooth extraction socket preservation is necessary due to alveolar bone
resorptive patterns through regenerative dentistry approaches that involve the use of stem
cells, scaffold and growth factor. Stem cells derived from human exfoliated deciduous teeth
(SHED) are known to potentially possess the osteogenic ability. Meanwhile, carbonate
apatite scaffold (CAS) can act as a biocompatible scaffold capable of supporting mesenchymal stem cells (MSCs) to proliferate and differentiate optimally. The aim of this study is to
investigate the expression of bone morphogenic protein-2 and 7 (BMP2, BMP7) and Matrix
Metalloproteinase-8 (MMP-8) after the transplantation of SHED-incorporated CAS during
in vivo bone remodeling.
Material and Methods: A total of 14 healthy, male, Wistar rats, whose mandible anterior teeth
were extracted by means of sterile needle holder clamps, constituted the subjects of this study of
alveolar bone defects. Two research groups were created: a control group (CAS) as group I and
an experimental group (CAS + SHED) as group II. SHED with a density of 106 cells were
incorporated into CAS before being transplanted into the experimental group. After 7 days, all
the animals were sacrificed and their mandible anterior region extracted. The BMP2, BMP7 and
MMP-8 expression were subsequently analyzed by means of immunostaining. An unpaired t-test
was conducted to analyze the treatment and control group (p<0.01) data.
Results: The expression of BMP-2 and BMP-7 was higher in group II compared to group
I. Meanwhile, the level of MMP-8 was lower in group II than group I. There was greater
significant increased expression of BMP-2 and BMP-7 expression in Group II compared to
Group I. There was significant decreased expression of MMP-8 between group II than group
I (p<0.01).
Conclusion: SHED-incorporated CAS can enhance BMP-2 and BMP-7 expression while
attenuating MMP-8 expression during in vivo alveolar bone remodeling. |
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