Gingival Mesenchymal Stem Cell Metabolite Decreasing TRAP, NFATc1, and Sclerotin Expression in LPS-Associated Inflammatory Osteolysis In Vivo

• Main Authors: Alexander Patera Nugraha, -, Nastiti Faradilla Ramadhani, -, Wibi Riawan, -, Igo Syaiful Ihsan, -, Diah Savitri Ernawati, -, Rini Devijanti Ridwan, -, Ida Bagus Narmada, -, Tania Saskianti, -, Fianza Rezkita, -, Andari Sarasati, -, Tengku Natasha Eleena Binti Tengku Ahmad Noor, -, Bilqis Inayatillah, -, Albertus Putera Nugraha, -, Florentina Joestandari, -
• Format: Article PeerReviewed
• Language: English; English; English; English
• Published: Georg Thieme Verlag 2022
• Subjects: RK Dentistry
• Online Access: https://repository.unair.ac.id/122729/1/19..pdf; https://repository.unair.ac.id/122729/2/19..pdf; https://repository.unair.ac.id/122729/3/19.pdf; https://repository.unair.ac.id/122729/4/19.Gingival.pdf; https://repository.unair.ac.id/122729/; https://www.thieme-connect.com/products/ejournals/pdf/10.1055/s-0042-1748529.pdf
• Institution: Universitas Airlangga

Objective Bone is a dynamic tissue that undergoes remodeling. During bone remodeling, there are transcription factors such as nuclear factor-activated T cells-1 (NFATc1), sclerostin, and tartrate-resistant acid phosphatase (TRAP) that are released for bone resorption. Metabolite from gingival mesenchymal stem cells (GMSCs) has the ability to activate proliferation, migration, immunomodulation, and tissue regeneration of bone cells and tissues. Furthermore, the aim of this study is to investigate the metabolite of GMSCs’ effect on expression of NFATc1, TRAP, and sclerostin in calvaria bone resorption of Wistar rats. Materials and Methods Twenty male healthy Wistar rats (Rattus norvegicus), 1 to 2 months old, 250 to 300 g body were divided into four groups, namely group 1 (G1): 100 µg phosphate-buffered saline day 1 to 7; group 2 (G2): 100 μg lipopolysaccharide (LPS) day 1 to 7; group 3 (G3): 100 μg LPS þ 100 μg GMSCs metabolite day 1 to 7; and group 4 (G4): 100 μg GMSCs metabolite day 1 to 7. Escherichia coli LPS was used to induce inflammatory osteolysis on the calvaria with subcutaneous injection. GMSCs metabolite was collected after passage 4 to 5, then injected subcutaneously on the