Osteoinduction Ability of Human Adipose-Derived Mesenchymal Stem Cell with Chitosan Scaffold Combination Towards Blood Serum Phosphorus Levels
ologous tissue can cause problems in the repair of extensive tissue damage, so the principle of tissue engineering is used as an alternative to reconstruct damage to the tissue because it has many advantages. The combination of Human Adipose-Derived Mesenchymal Stem Cell (hADMSC) and chitosan sca...
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id-langga.1236902023-04-17T00:40:54Z https://repository.unair.ac.id/123690/ Osteoinduction Ability of Human Adipose-Derived Mesenchymal Stem Cell with Chitosan Scaffold Combination Towards Blood Serum Phosphorus Levels Dian Agustin Wahjuningrum, - Nindya R A Marhendra, - M. Roelianto, - Ari Subiyanto, - Irmaleny, - Fery Setiawan, - Syania E Febriyanti, - Setyabudi, - Tamara Yuanita, - Swadheena Patro, - Anuj Bhardwaj, - RK Dentistry ologous tissue can cause problems in the repair of extensive tissue damage, so the principle of tissue engineering is used as an alternative to reconstruct damage to the tissue because it has many advantages. The combination of Human Adipose-Derived Mesenchymal Stem Cell (hADMSC) and chitosan scaffold is expected to trigger osteoinduction that can be expressed by osteogenic markers such as phosphorus levels in blood serum. Objectives to prove osteoinduction in a combination of hADMSC and chitosan scaffold using blood serum phosphorus levels. This study used 12 groups with 5 sample each. Groups 1 to 4 were the negative control group at day 1,3,7, and 14. While groups 5 to 8 were the positive control group at day 1,3,7, and 14. Groups 9 to 12 were treatment groups at day 1,3,7, and 14. In the negative control group bone was only removed, in positive control group, bone was removed and chitosan scaffold was added, and in treatment group, bone was removed then, hADMSC and chitosan scaffold combination was added. Blood collection will be carried out in each group for examination of phosphorus levels in the blood serum. There were differences in phosphorus levels in blood serum in each group even though statistically there were only significant differences on day 14. The combination of hADMSC and chitosan scaffold caused a significant change in blood serum phosphorus levels on day 14 which means it triggers osteoinduction. 2021 Article PeerReviewed text en https://repository.unair.ac.id/123690/1/5.pdf text en https://repository.unair.ac.id/123690/2/5.pdf text en https://repository.unair.ac.id/123690/3/5.pdf Dian Agustin Wahjuningrum, - and Nindya R A Marhendra, - and M. Roelianto, - and Ari Subiyanto, - and Irmaleny, - and Fery Setiawan, - and Syania E Febriyanti, - and Setyabudi, - and Tamara Yuanita, - and Swadheena Patro, - and Anuj Bhardwaj, - (2021) Osteoinduction Ability of Human Adipose-Derived Mesenchymal Stem Cell with Chitosan Scaffold Combination Towards Blood Serum Phosphorus Levels. Journal of International Dental and Medical Research. |
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RK Dentistry Dian Agustin Wahjuningrum, - Nindya R A Marhendra, - M. Roelianto, - Ari Subiyanto, - Irmaleny, - Fery Setiawan, - Syania E Febriyanti, - Setyabudi, - Tamara Yuanita, - Swadheena Patro, - Anuj Bhardwaj, - Osteoinduction Ability of Human Adipose-Derived Mesenchymal Stem Cell with Chitosan Scaffold Combination Towards Blood Serum Phosphorus Levels |
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ologous tissue can cause problems in the repair of extensive tissue damage, so the principle of
tissue engineering is used as an alternative to reconstruct damage to the tissue because it has
many advantages. The combination of Human Adipose-Derived Mesenchymal Stem Cell
(hADMSC) and chitosan scaffold is expected to trigger osteoinduction that can be expressed by
osteogenic markers such as phosphorus levels in blood serum.
Objectives to prove osteoinduction in a combination of hADMSC and chitosan scaffold using blood
serum phosphorus levels. This study used 12 groups with 5 sample each. Groups 1 to 4 were the
negative control group at day 1,3,7, and 14. While groups 5 to 8 were the positive control group at
day 1,3,7, and 14. Groups 9 to 12 were treatment groups at day 1,3,7, and 14. In the negative
control group bone was only removed, in positive control group, bone was removed and chitosan
scaffold was added, and in treatment group, bone was removed then, hADMSC and chitosan
scaffold combination was added.
Blood collection will be carried out in each group for examination of phosphorus levels in the blood
serum. There were differences in phosphorus levels in blood serum in each group even though
statistically there were only significant differences on day 14. The combination of hADMSC and
chitosan scaffold caused a significant change in blood serum phosphorus levels on day 14 which
means it triggers osteoinduction. |
format |
Article PeerReviewed |
author |
Dian Agustin Wahjuningrum, - Nindya R A Marhendra, - M. Roelianto, - Ari Subiyanto, - Irmaleny, - Fery Setiawan, - Syania E Febriyanti, - Setyabudi, - Tamara Yuanita, - Swadheena Patro, - Anuj Bhardwaj, - |
author_facet |
Dian Agustin Wahjuningrum, - Nindya R A Marhendra, - M. Roelianto, - Ari Subiyanto, - Irmaleny, - Fery Setiawan, - Syania E Febriyanti, - Setyabudi, - Tamara Yuanita, - Swadheena Patro, - Anuj Bhardwaj, - |
author_sort |
Dian Agustin Wahjuningrum, - |
title |
Osteoinduction Ability of Human Adipose-Derived Mesenchymal Stem Cell
with Chitosan Scaffold Combination Towards Blood Serum Phosphorus Levels |
title_short |
Osteoinduction Ability of Human Adipose-Derived Mesenchymal Stem Cell
with Chitosan Scaffold Combination Towards Blood Serum Phosphorus Levels |
title_full |
Osteoinduction Ability of Human Adipose-Derived Mesenchymal Stem Cell
with Chitosan Scaffold Combination Towards Blood Serum Phosphorus Levels |
title_fullStr |
Osteoinduction Ability of Human Adipose-Derived Mesenchymal Stem Cell
with Chitosan Scaffold Combination Towards Blood Serum Phosphorus Levels |
title_full_unstemmed |
Osteoinduction Ability of Human Adipose-Derived Mesenchymal Stem Cell
with Chitosan Scaffold Combination Towards Blood Serum Phosphorus Levels |
title_sort |
osteoinduction ability of human adipose-derived mesenchymal stem cell
with chitosan scaffold combination towards blood serum phosphorus levels |
publishDate |
2021 |
url |
https://repository.unair.ac.id/123690/1/5.pdf https://repository.unair.ac.id/123690/2/5.pdf https://repository.unair.ac.id/123690/3/5.pdf https://repository.unair.ac.id/123690/ |
_version_ |
1764209104365027328 |