Phytochemical Analysis and Antibacterial Activity of Purple Leaf Extract [Graptophyllum pictum (L.) Griff] Against Streptococcus mutans
Background. Streptococcus mutans is the leading cause of dental caries. One of many medicinal plants, purple leaf [Graptophyllum pictum (L.) Griff], which contains flavonoids, alkaloids, tannins, steroids, and saponins, is a potential antibacterial agent. Objective. This study aimed to determine...
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id-langga.1237272023-04-17T00:57:31Z https://repository.unair.ac.id/123727/ Phytochemical Analysis and Antibacterial Activity of Purple Leaf Extract [Graptophyllum pictum (L.) Griff] Against Streptococcus mutans Devi Eka Juniarti, - Tuti Kusumaningsih, - Wisnu Setyari Juliastuti,, - Adioro Soetojo, - Nabilla Dara Wungsu, - RK Dentistry Background. Streptococcus mutans is the leading cause of dental caries. One of many medicinal plants, purple leaf [Graptophyllum pictum (L.) Griff], which contains flavonoids, alkaloids, tannins, steroids, and saponins, is a potential antibacterial agent. Objective. This study aimed to determine the antibacterial activity of purple leaf extract (Graptophyllum pictum L. Griff) against Streptococcus mutans. Methods. Streptococcus mutans were suspended in several Graptophyllum pictum (L.) Griff extract concentrations in a BHIB medium using the dilution method so that the concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78% were obtained. Each tube was incubated for 24 hours, then subcultured in a Tryptone Yeast Extract Cystine medium in a petri dish using a spreader. Each petri dish was set for 24 hours; the growth of the colony, using CFU/mL unit, was manually calculated. The samples were then subjected to microbiological analysis. The Tukey's Honest Significant Difference test was performed to determine if the relationship between the sets of data in the treatment group is statistically significant (p<0.05). Results. Purple leaf extract contains bioactive compounds such as flavonoid, alkaloid, tannin, triterpenoid/ steroid, and saponin. The Minimum Inhibitory Concentration (MIC) of Graptophyllum pictum (L.) Griff against Streptococcus mutans was in concentration 3.125%, and the Minimum Bactericidal Concentration (MBC) was in concentration 6.25%. Conclusion. Purple Leaf Extract [Graptophyllum pictum (L.) Griff] has antibacterial activity against Streptococcus mutans. 2021 Article PeerReviewed text en https://repository.unair.ac.id/123727/1/2.pdf text en https://repository.unair.ac.id/123727/2/2.pdf text en https://repository.unair.ac.id/123727/3/2.pdf text en https://repository.unair.ac.id/123727/4/2.pdf Devi Eka Juniarti, - and Tuti Kusumaningsih, - and Wisnu Setyari Juliastuti,, - and Adioro Soetojo, - and Nabilla Dara Wungsu, - (2021) Phytochemical Analysis and Antibacterial Activity of Purple Leaf Extract [Graptophyllum pictum (L.) Griff] Against Streptococcus mutans. Acta Medica Philippina. |
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RK Dentistry Devi Eka Juniarti, - Tuti Kusumaningsih, - Wisnu Setyari Juliastuti,, - Adioro Soetojo, - Nabilla Dara Wungsu, - Phytochemical Analysis and Antibacterial Activity of Purple Leaf Extract [Graptophyllum pictum (L.) Griff] Against Streptococcus mutans |
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Background. Streptococcus mutans is the leading cause of dental caries. One of many medicinal plants, purple
leaf [Graptophyllum pictum (L.) Griff], which contains flavonoids, alkaloids, tannins, steroids, and saponins, is a
potential antibacterial agent.
Objective. This study aimed to determine the antibacterial activity of purple leaf extract (Graptophyllum pictum
L. Griff) against Streptococcus mutans.
Methods. Streptococcus mutans were suspended in several Graptophyllum pictum (L.) Griff extract concentrations in
a BHIB medium using the dilution method so that the concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.12%,
1.56%, 0.78% were obtained. Each tube was incubated for 24 hours, then subcultured in a Tryptone Yeast Extract
Cystine medium in a petri dish using a spreader. Each petri dish was set for 24 hours; the growth of the colony,
using CFU/mL unit, was manually calculated. The samples were then subjected to microbiological analysis. The
Tukey's Honest Significant Difference test was performed to determine if the relationship between the sets of data
in the treatment group is statistically significant (p<0.05).
Results. Purple leaf extract contains bioactive compounds such as flavonoid, alkaloid, tannin, triterpenoid/
steroid, and saponin. The Minimum Inhibitory Concentration (MIC) of Graptophyllum pictum (L.) Griff against
Streptococcus mutans was in concentration 3.125%, and the Minimum Bactericidal Concentration (MBC) was in
concentration 6.25%.
Conclusion. Purple Leaf Extract [Graptophyllum pictum (L.) Griff] has antibacterial activity against Streptococcus
mutans. |
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Article PeerReviewed |
author |
Devi Eka Juniarti, - Tuti Kusumaningsih, - Wisnu Setyari Juliastuti,, - Adioro Soetojo, - Nabilla Dara Wungsu, - |
author_facet |
Devi Eka Juniarti, - Tuti Kusumaningsih, - Wisnu Setyari Juliastuti,, - Adioro Soetojo, - Nabilla Dara Wungsu, - |
author_sort |
Devi Eka Juniarti, - |
title |
Phytochemical Analysis and Antibacterial Activity of
Purple Leaf Extract [Graptophyllum pictum (L.) Griff]
Against Streptococcus mutans |
title_short |
Phytochemical Analysis and Antibacterial Activity of
Purple Leaf Extract [Graptophyllum pictum (L.) Griff]
Against Streptococcus mutans |
title_full |
Phytochemical Analysis and Antibacterial Activity of
Purple Leaf Extract [Graptophyllum pictum (L.) Griff]
Against Streptococcus mutans |
title_fullStr |
Phytochemical Analysis and Antibacterial Activity of
Purple Leaf Extract [Graptophyllum pictum (L.) Griff]
Against Streptococcus mutans |
title_full_unstemmed |
Phytochemical Analysis and Antibacterial Activity of
Purple Leaf Extract [Graptophyllum pictum (L.) Griff]
Against Streptococcus mutans |
title_sort |
phytochemical analysis and antibacterial activity of
purple leaf extract [graptophyllum pictum (l.) griff]
against streptococcus mutans |
publishDate |
2021 |
url |
https://repository.unair.ac.id/123727/1/2.pdf https://repository.unair.ac.id/123727/2/2.pdf https://repository.unair.ac.id/123727/3/2.pdf https://repository.unair.ac.id/123727/4/2.pdf https://repository.unair.ac.id/123727/ |
_version_ |
1764209110360784896 |