Biomodulator of Diode Laser Irradiation on Odontoblast-Like Cells by Expression of Vascular Endothelial Growth Factor-A and Transforming Growth Factor-β1
Objective This study aimed to prove that the effect of diode laser 650-nm irradiation to the expression of vascular endothelial growth factor (VEGF)-A and transforming growth factor (TGF)-β1 plays important roles in dental pulp-regulating cell proliferation, differentiation, and revascularization....
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| Main Authors: | , , , |
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| Format: | Article PeerReviewed |
| Language: | English English English |
| Published: |
2022
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| Subjects: | |
| Online Access: | https://repository.unair.ac.id/123730/1/5.pdf https://repository.unair.ac.id/123730/2/5.pdf https://repository.unair.ac.id/123730/3/5.pdf https://repository.unair.ac.id/123730/ |
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| Institution: | Universitas Airlangga |
| Language: | English English English |
| Summary: | Objective This study aimed to prove that the effect of diode laser 650-nm irradiation
to the expression of vascular endothelial growth factor (VEGF)-A and transforming
growth factor (TGF)-β1 plays important roles in dental pulp-regulating cell proliferation, differentiation, and revascularization.
Materials and Methods The research was performed by randomized posttest only
control group design using Rattus norvegicus. A total of 48 samples were provided and
divided into eight groups of 6 samples each with a random-sample allocation. Each
group were prepared, and perforation of maxillary first molar were done. In control
groups (groups 1–4), glass ionomer cement (GIC) was used to restore the teeth, while
in laser groups (groups 5–8), the teeth were irradiated with diode laser 650 nm for
40 seconds before application of GIC. Half of the groups (groups 1, 2, 5, and 6) were
necropsied in 7 days, and the rest (groups 3, 4, 7, and 8) were necropsied in 14 days.
Immunohistochemistry (IHC) evaluation were implemented to check the expression of
both VEGF-A and TGF-β1.
Statistical Analysis Both data of VEGF-A and TGF-β1 expression were analyzed using a
one-way ANOVA (α ¼ 0.05) with SPSS statistical software.
Results The study showed that the diode laser 650-nm irradiation increased expression of
VEGF-A and TGF-β1, and there was a significant difference between diode laser and control
group on VEGF-A expression (p¼ 0.001) and TGF- β1 (p ¼ 0.000) on days 7 and 14.
Conclusion Diode laser 650 nm with 40-second irradiation time shows increment
from day 7 to day 14 reflecting increase in pulp healing by modulating VEGF-A and TGF-
β1 expression since days 7 to 14. |
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