SITOTOXICITY TEST OF NEEM LEAVES (Azadirachta indica) TOWARDS BHK-21 FIBROBLAST CEL
Background: The leaves of neem (Azadirachta indicia) is one of herbal medicine that recommended as an alternative material of root canal irrigants. The active ingredients of neem leaves such as alkaloids, tannins, saponins and flavonoids has been proven to have antibacterial activity against E. fa...
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| Main Authors: | , , |
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| Format: | Article PeerReviewed |
| Language: | English English English |
| Published: |
2017
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| Subjects: | |
| Online Access: | https://repository.unair.ac.id/123740/1/11.pdf https://repository.unair.ac.id/123740/2/11.pdf https://repository.unair.ac.id/123740/3/11.pdf https://repository.unair.ac.id/123740/ |
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| Institution: | Universitas Airlangga |
| Language: | English English English |
| Summary: | Background: The leaves of neem (Azadirachta indicia) is one of herbal medicine that recommended as an
alternative material of root canal irrigants. The active ingredients of neem leaves such as alkaloids, tannins,
saponins and flavonoids has been proven to have antibacterial activity against E. faecalis. The ideal
properties of an alternative material of root canal irrigants is not only have antibacterial activity but also is
not toxic to the tissues, but the toxicity of neem leaves remains unclear until now. Objective: The aims of this
study to determine the toxicity effect of neem leaves extract in specific concentration. Methods: This
research was an experimental laboratory in vitro study of baby hamster kidney fibroblast (BHK-21). The
neem leaves extract was made by maceration method using ethanol 96% and certain dilution performed to
obtain various concentrations. Cytotoxicity test was conducted by MTT assay and the optical density was
measured using ELISA reader at wavelength of 620 nm. Then, the optical density values were calculated
using the formula for determining the number of survival fibroblasts after tested. Results: The percentage of
survival fibroblast at concentration of 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95, and 100% in
sequence were 71.3%, 64, 2%, 62%, 60.2%, 40.1%, 36.3%, 36.7%, 29%, 28.4%, 27.5%, and 25.6% .
Conclusion: The extract of neem leaves (Azadirachta indica) has cytotoxic effects at concentration of 70%
up to 100%. |
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