Metagenomic profiles of core and signature bacteria in the guts of white shrimp, Litopenaeus vannamei, with different growth rates
A large number of studies have previously revealed evidence suggesting a strong correlation between gut bacteria and the growth performance of aquatic animals. However, specific research reporting bacterial communities inhabiting white shrimps with different growth rates are still very limited. Th...
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Main Authors: | , , , , |
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Format: | Article PeerReviewed |
Language: | English English English English |
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ELSEVIER
2022
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Online Access: | https://repository.unair.ac.id/123816/1/C1.4.pdf https://repository.unair.ac.id/123816/2/C1.4.pdf https://repository.unair.ac.id/123816/5/C1.1-32%20Karil%20Jurnal%20Ilmiah-4.pdf https://repository.unair.ac.id/123816/6/4-3.%20SK%20No.%20961-4.pdf https://repository.unair.ac.id/123816/ https://www.sciencedirect.com/science/article/pii/S004484862101512X https://doi.org/10.1016/j.aquaculture.2021.737849 |
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Institution: | Universitas Airlangga |
Language: | English English English English |
Summary: | A large number of studies have previously revealed evidence suggesting a strong correlation between gut bacteria
and the growth performance of aquatic animals. However, specific research reporting bacterial communities
inhabiting white shrimps with different growth rates are still very limited. Thus, the present study aimed at
investigating the structure and composition of core and signature bacteria in the gut of shrimps at different
growth rates. A total of 60 healthy white shrimps, Litopenaeus vannamei, with different growth rates (slow vs fast)
were collected from three shrimp ponds as replicates. Bacterial 16S rRNA genes associated with these shrimp guts
were extracted, amplified and sequenced using next-generation sequencing (NGS) analysis to determine the
structure and composition of the bacterial community within and between groups. The result showed that 35
genera of 181 detected genera (19.34%) were considered to be the core microbiome in the gut of white shrimps
regarding their prevalence in all samples including Illumatobacter, Ruegeria, Candidatus Bacillopora, Roseovarius,
Silicimonas, Algoriphagus, Haloferula, Dinoroesobacter, Vibrio, Lactobacillus, Bdellovibrio, Shimia, and
Robiginitalea. In addition, there was a strong association between diversity and species richness of gut bacteria
and the growth of white shrimps. The species richness and the Shannon index representing bacterial diversity
were significantly lower in the fast-growing shrimps (p < 0.05), reinforcing the close relationship between gut
bacteria and their host growth. Further analysis using linear discriminant analysis effect size (LEfSe) indicated
that nine bacterial species were significantly higher in the fast-growing shrimps (Group 1) than the slow-growing
shrimps (Group 2). The nine species were identified as Coprococcus comes (OTU58), Oscillibater sp. ER4 (OTU74),
Acidaminicoccus intestini (OTU210) and Bacteroidetes ovatus (OTU671), Oscilibacter sp. (OTU274), Peptococcus sp.
(OTU218), Clostridium phoceensis (OTU313), Legionella sp. (OTU682), and unidentified Clostridiales (OTU186).
These results might suggest that the nine bacterial species are bacterial signatures for the high growth shrimps.
However, due to the novelty of the shrimp gut bacteria, further studies are still required to understand their
specific roles and contribution to the growth of white shrimps. |
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