Biodegrable Collagen, Hydroxyapatite, and Epigallocatechin-3-Gallate Hydrogel Scaffold as an Induction Material for Pulp Dentin Regenaration

• Main Authors: Elline, -, Kun Ismiyatin, -, Theresia Indah Budhy S., -
• Format: Article PeerReviewed
• Language: English; English; English; English
• Published: 2023
• Subjects: RK Dentistry
• Online Access: https://repository.unair.ac.id/123876/1/5.pdf; https://repository.unair.ac.id/123876/2/5.pdf; https://repository.unair.ac.id/123876/5/5.pdf; https://repository.unair.ac.id/123876/7/Biodegradable.pdf; https://repository.unair.ac.id/123876/
• Institution: Universitas Airlangga

Introduction: Current regenerative endodontic treatment approaches preserve pulp vitality and use tissue engineering concepts. One of the essential factors in pulp tissue engineering is a scaffold. In several reviews, direct bioactive material applied to the pulp without using scaffold will cause the temporary release, which was unstable. The scaffold can increase the success of pulp vital therapy treatment because the scaffold can facilitate stem cells to adhere, proliferate, differentiate and support regeneration. Hydrogel scaffold is considerable because it can mimicks extracellular matrix (ECM). It should have several essential characterizations, and one of them is biodegradable ability. New composite hydrogel scaffolds were developed as an organic and inorganic material hybrid. Objective: To compare the biodegradation value of Col-HA-EGCG hydrogel scaffold on days 3 and 7 after immersion. Materials and Methods: Samples were synthesized with the mixing of 1% hydroxyapatite solution and collagen solution until homogen, added 10 µmol/L EGCG into the solution. After that, 2% HPMC was used to stable the gelling process. Samples were freeze-dried for 24 hours and immersed in Phosphate Buffer Salin containing 1,6µg/ml of lysozyme enzyme. The degradation value percentages determined by measuring the difference weight of dry scaffold before and after immersion. Results: The data were analyzed by T-test, and it showed the Col-HA-EGCG hydrogel scaffold can be degraded, and there were no significant biodegradation values in 3 and 7 days. Conclusions: The Col-HAEGCG is biodegradable in lysozyme enzyme. The biodegradation rate on Col-HA-EGCG scaffold on 3 and 7 days were not significant.