In vitro evaluation of FGF-2 on osteoblast cell seeded in nano chitosancarbonate apatite chitosan scaffold
Background: Scaffold is a medium for extracellular matrix synthesis and Becomes a place for attachment and growth of new cells. Some polymeric materials, the which have been developed in tissue engineering are chitosan. Chitosan is one of the best materials used in tissue engineering due to its b...
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| Main Authors: | , , , , |
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| Format: | Article PeerReviewed |
| Language: | English English English |
| Published: |
2017
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| Subjects: | |
| Online Access: | https://repository.unair.ac.id/124151/1/8.pdf https://repository.unair.ac.id/124151/2/8.pdf https://repository.unair.ac.id/124151/3/8.pdf https://repository.unair.ac.id/124151/ |
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| Institution: | Universitas Airlangga |
| Language: | English English English |
| Summary: | Background: Scaffold is a medium for extracellular matrix synthesis and Becomes a place for
attachment and growth of new cells. Some polymeric materials, the which have been
developed in tissue engineering are chitosan. Chitosan is one of the best materials used in
tissue engineering due to its biodegradable, biocompability, anti-bacterial properties, for
wound healing and bioadded characters. The carbonate apatite material is used as a
biocompatible material for medical purposes. Carbonate apatite Increased material the
chemical bonds between biomaterials and bone tissue that can Affect bone formation. The
nanostructured biomaterials Significantly Enhance cell functioning leading to Increased
osteoinductivity and oseointegrativity. So based on the explanation and understanding of the
mechanisms of the relationship between the bone regeneration process and the nano chitosancarbonate
apatite scaffold, it is important to improve the method for the next generation of
scaffolds that can demonstrate bone efficiency and regeneration in vitro. Objective: To
Determine the Increased expression of FGF-2 on osteoblast cell planting in nano chitosancarbonate
apatite scaffold and the results of this study are expected to be used as a selection
method of bone graft using nano-chitosan scaffold. Methods: Laboratory experimental study
with post test only group design on seven samples, the osteoblast cells planted on chitosan
nano-carbonate apatite scaffold with observation time on day 3, day 5, and day 7 and
Increased expression of FGF- 2 osteoblast cells were measured by Immunohistochemistry
(IHC) method. The results were Analyzed by statistical test of multiple comparison test of Post
Hoc and Tukey test. Result: The results Showed a significant difference (P <0.05) between
treatment groups in the observation time on day 3, 5 and 7. Conclusion: The experiment has
shown that the increase is of FGF-2 expression in osteoblast cells planted in chitosan nanocarbonate
apatite scaffold has Increased in observation time on day 3, 5 and 7. |
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