Expression of NF-κB and MMP-7 on Defenses of the Gingival Epithelium Injected LPS Porphyromonas Gingivalis with the Administration of Curcumin

• Main Authors: Eka Fitria Augustina, -, Ketut Sudiana, -, Soetjipto, -, Muhammad Rubianto, -
• Format: Article PeerReviewed
• Language: English; English; English; English; English
• Published: 2019
• Subjects: RK Dentistry
• Online Access: https://repository.unair.ac.id/124502/1/1.pdf; https://repository.unair.ac.id/124502/2/1.pdf; https://repository.unair.ac.id/124502/3/karil%201.pdf; https://repository.unair.ac.id/124502/4/korespomden.pdf; https://repository.unair.ac.id/124502/9/KORESPONDEN.pdf; https://repository.unair.ac.id/124502/
• Institution: Universitas Airlangga

Epithelial cells in the gingiva epithelium are among the first host cells encountered by periodontal pathogens such as Porphyromonas gingivalis. The lipopolysaccharide (LPS) of P. gingivalis is a crucial virulence factor critically involved in the regulation of immune inflammatory responses. Early prevention is necessary for the onset of periodontitis, especially in tissues that have been exposed to gingivitis by maintaining the primary defenses of the gingival epithelium. One of the effective and efficient efforts by utilizing the plant nutritious plant extracts curcumin. Curcumin is a medicinal plant material that has anti-inflammatory properties, as a factor inhibiting transcription factor NF-κB. Barriers to NF-κB are expected to decrease the expression of pro-inflammatory mediators that play a role in defective gingival epithelial defenses in cases of gingivitis. It is therefore suspected that inflammatory mediators associated with periodontitis, NF-κB and MMP-7 also play an important role in the mechanism of damage to the gingival epithelial defect which may result in the breakdown of deeper periodontal tissue. The aim of the study is to determine the role of NF-κB and MMP-7 in the defense of the gingival epithelium in intervention by LPS Porphyromonas gingivalis by administering curcumin. Materials and methods: 40 wistar rats, aged 2-3 months divided into control and treatment group. which got 5 heads for each group. Animal production of gingivitis, performed by induction of LPS P.gingivalis is injected into the gingival sulcus of the mandibular incisor proximal portion and since 48 hours from injection of LPS P gingivalis done giving kurkumin. Days 1, 7, and 14, wistar is sacrificed according to the group and gingival epithelial tissue is taken for immunohistochemical examination procedure to determine expressions of NFKβ and MMP-7. The results showed that administration of curcumin in wistar rats after induction with LPS P. gingivalis decreased NF-κB and MMP-7 expression. Decreased expression of inflammatory mediators can keep the gingival epithelial defenses.