The Effect of conditioned-Medium Human Adipose-Derived Mesenchymal Stem Cell In Apoptosis Of Bladder Cancer Cells

• Main Authors: Ari Alauddin Mawdudi, Ari, Furqan Hidayatullah, Furqan, Indra Bachtiar, Indra, Arif Rachman, Arif, Indri Lakhsmi Putri, Indri, Fabio Castiglione, Fabio, Wahjoe Djatisoesanto, Wahjoe, Lukman Hakim, Lukman
• Format: Article PeerReviewed
• Language: English; Indonesian; English; Indonesian; English
• Published: Indonesian Urological Association
• Subjects: R5-920 Medicine (General)
• Online Access: https://repository.unair.ac.id/124815/1/16%20artikel.pdf; https://repository.unair.ac.id/124815/2/16.%20karil.pdf; https://repository.unair.ac.id/124815/3/16.%20Korespondensi.pdf; https://repository.unair.ac.id/124815/4/16.%20Etik.pdf; https://repository.unair.ac.id/124815/5/16.%20turnitin.pdf; https://repository.unair.ac.id/124815/; http://juri.urologi.or.id/juri/article/view/653; https://doi.org/10.32421/juri.v28i1.653
• Institution: Universitas Airlangga

Abstract Objective: To determine the effect of conditioned medium human Adipose-Derived Mesenchymal Stem Cells (CM-hADMSC) on apoptosis of urothelial bladder cancer cells. Material & Methods: Bladder (5637) cancer cell lines cultured in conditioned media harvested from human adipose-derived mesenchymal stem cells (hADMSC). Flow cytometry tests were carried out using the Flowcytometry Acquisition cell sorting (FACS) Calibur to measure apoptosis. Results: There was a significant difference in the percentage of late apoptosis in the group receiving culture medium treatment: CM-hADMSC 1: 1 to the entire study group. Further analysis revealed no difference in the average percentage of late apoptosis in groups exposed to culture medium: CM-hADMSC 1: 2 and culture medium: CM-hADMSC 1: 4 (p> 0.05). Conclusion: CM-hADMSC at a 1: 1 dose concentration to culture medium obtain a significant increase of apoptosis in bladder cancer cells.