The Effect Of Conditioned Medium Adipose Derived Mesenchymal Stem Cells In Urothelial Carcinoma Culture Cells Viability
Abstract Objective: This study aimed to gives a perspective in CM-ADMSCs effect in urothelial bladder cancer viability. Material & Methods: Human bladder cell carcinoma type 5637 was used as the subject of this in vitro study. This study contains four different groups: untreated control group,...
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| Main Authors: | , , , , , , , |
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| Format: | Article PeerReviewed |
| Language: | English Indonesian English Indonesian English |
| Published: |
Indonesian Urological Association
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| Subjects: | |
| Online Access: | https://repository.unair.ac.id/124822/1/17%20artikel.pdf https://repository.unair.ac.id/124822/2/17.%20karil.pdf https://repository.unair.ac.id/124822/3/17.%20Korespondensi.pdf https://repository.unair.ac.id/124822/4/17.%20Etik.pdf https://repository.unair.ac.id/124822/5/17.%20turnitin.pdf https://repository.unair.ac.id/124822/ http://juri.urologi.or.id/juri/article/view/654 https://doi.org/10.32421/juri.v28i1.654 |
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| Institution: | Universitas Airlangga |
| Language: | English Indonesian English Indonesian English |
| Summary: | Abstract
Objective: This study aimed to gives a perspective in CM-ADMSCs effect in urothelial bladder cancer viability. Material & Methods: Human bladder cell carcinoma type 5637 was used as the subject of this in vitro study. This study contains four different groups: untreated control group, Culture medium: hADMSCs with 1:1, 1:2, and 1:4 concentration group. Each group consists of 6 replications to prevent bias of the study. Viability was determined with MTT assay methods and evaluation performed after 48 h exposure of conditioned medium. Results: A post hoc test was conducted to analyze the data. The 5637 bladder cancer cell line demonstrated significantly decreased viability after exposure to culture medium: CM-hADMSCs 1:1 (p: 0.002) compared to the negative control group, but there are no significant differences in viability between the control groups with groups that were exposed to culture medium: CM-hADMSCs 1:2 and culture medium: CM-hADMSCs 1:4 with p: 0.480 and p: 0.060 respectively. Conclusion: Decreased viability of urothelial bladder cancer cells after exposure to CM-hADMSCs occurs at a concentration of 1:1 and Dosage addition more than 1:1 concentration doesn’t give any advantages. |
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