The Effect of Limbal Mesenchymal Stem Cell Secretome on Corneal Expression of Interleukin-1 in Experimental Model of Corneal Alkali Injury

• Main Authors: Okiningtyas Kun Cahyandari, -, Evelyn Komaratih, -, Wimbo Sasono, -, Djoko Legowo, -, Hari Basuki Notobroto, -
• Format: Article PeerReviewed
• Language: English; English; English
• Published: International Journal of Research Publications (IJRP) 2022
• Subjects: R Medicine
• Online Access: https://repository.unair.ac.id/124966/1/C99_Artikel.pdf; https://repository.unair.ac.id/124966/2/C99_Turnitin.pdf; https://repository.unair.ac.id/124966/3/C99_Validasi.pdf; https://repository.unair.ac.id/124966/; https://www.ijrp.org/; https://doi.org/10.47119/IJRP100951220222915
• Institution: Universitas Airlangga

Background: To investigate the effect of limbal mesenchymal stem cells secretome (L-MSCS) on corneal expression of Interleukin-1 (IL-1) in alkali ocular chemical injury animal model. Methods: Secretome was collected from limbal mesenchymal stem cells of corneoscleral tissue of healthy male rabbit. Rabbits were exposed to NaOH. The research subjects consisted of 3 groups, normal control group, Balance saline solution (BSS) group, and L-MSCS group. The level of corneal expression of IL-1 by immunohistochemical staining were analyzed. Results: After seven days administration of topical instillation of L-MSCS, the mean level of IL-1 expression with immunoreactive score (IRS) was 3,2 in normal group, 5,4 in BSS group, and 5,1 in L-MSCS group. The level of IL-1 expression in L-MSCS group was lower than BSS group. There was no difference in IL-1 expression between L-MSCS group and the BSS group (p>0.05) but a significant difference was found between normal group and BSS group, normal group and L-MSCS group, and between the three groups (p<0.05). Conclusions: Topical instillation of L-MSCS has a potential effect on decrease the level of IL-1 expression in the 7th day of chemical ocular injury animal model even though the difference was not statistically significant.