Assessment of fish biodiversity in four Korean rivers using environmental DNA metabarcoding
Environmental DNA (eDNA) metabarcoding is a cost-effective novel approach to estimate biodiversity in an ecosystem. In this study, the MiFish pipeline was employed to test if the system methodology is sufficiently reliable to estimate fish biodiversity in Korean rivers. A total of 125 unique hapl...
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Main Authors: | , , , , , |
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Format: | Article PeerReviewed |
Language: | English English English English |
Published: |
Peer Journal - Biodiversity and Conservation
2016
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Subjects: | |
Online Access: | https://repository.unair.ac.id/125106/1/12.pdf https://repository.unair.ac.id/125106/2/12%20T.pdf https://repository.unair.ac.id/125106/5/13.pdf https://repository.unair.ac.id/125106/6/ethics%20sapto1.pdf https://repository.unair.ac.id/125106/ https://peerj.com/articles/9508/ http://dx.doi.org/10.7717/peerj.9508 |
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Institution: | Universitas Airlangga |
Language: | English English English English |
Summary: | Environmental DNA (eDNA) metabarcoding is a cost-effective novel approach to
estimate biodiversity in an ecosystem. In this study, the MiFish pipeline was employed
to test if the system methodology is sufficiently reliable to estimate fish biodiversity
in Korean rivers. A total of 125 unique haplotypes and 73 species were identified
at the species level from 16 water samples collected from a single survey in four
Korean rivers (Hyeongsan, Taehwa, Seomjin, and Nakdong). Among the four rivers,
the highest species richness was recorded in the Seomjin River (52 species), followed
by the Taehwa (42 species) and Hyeongsan (40 species) rivers. The Nakdong River
(26 species) presented the lowest species richness and number of endemic species,
presumably due to its metropolitan location and anthropogenic impacts, such as dams
or weirs. We were also able to detect that five exotic species (Carassius cuvieri, Cyprinus
carpio, Cyprinus megalophthalmus, Lepomis macrochirus, and Micropterus salmoides)
are widely distributed in all surveyed rivers, a situation that might be problematic in
terms of conservation. Our findings indicate that the eDNA metabarcoding technique
is one of the most cost-effective scientific tools available for the management and
conservation of the freshwater fish resources available in Korea. However, the low
number of 12S sequences of endemic species in the database and low resolution of
the MiFish region for differentiating several taxa should be upgraded for their wide
use. |
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