Inactivation of HIV-1 Infection through Integrative Blocking with Amino Phenylboronic Acid Attributed Carbon Dots

• Main Authors: Yu Yu Aung, .-, Alfinda Novi Kristanti, .-, Siti Qamariyah Khairunisa, .-, Nasronudin Nasronudin, .-, Mochamad Zakki Fahmi*, .-
• Format: Article PeerReviewed
• Language: English; English; English; English
• Published: American Chemical Society (ACS) 2020
• Subjects: Q Science; QD Chemistry; QD450-801 Physical and theoretical chemistry
• Online Access: https://repository.unair.ac.id/125917/1/C10.%20Fulltext_.pdf; https://repository.unair.ac.id/125917/2/C10.%20Penilaian%20dan%20Validasi.pdf; https://repository.unair.ac.id/125917/4/C10.%20Similarity.pdf; https://repository.unair.ac.id/125917/3/C10%20Inactivation%20of%20HIV-1%20Infection.pdf; https://repository.unair.ac.id/125917/; https://pubs.acs.org/doi/pdf/10.1021/acsbiomaterials.0c00508
• Institution: Universitas Airlangga

Current antiretroviral HIV therapies continue to have problems related to procedural complications, toxicity, and uncontrolled side effects. In this study, amino phenylboronic acid-modified carbon dots (APBA-CDs) were introduced as a new nanoparticle-based on gp120 targeting that inhibits HIV-1 entry processes. Prolonged by simple pyrolysis for preparing carbon dots, this report further explores attributing amino phenylboronic acid on carbon dots, which prove the formation of graphene-like structures on carbon dots and boronic acid sites, thereby enabling the enhancement of positive optical properties through photoluminescent detection. Aside from performing well in terms of biocompatibility and low cytotoxicity (the CC50 reach up to 11.2 mg/mL), APBA-CDs exhibited superior capabilities in terms of prohibiting HIV-1 entry onto targeted MOLT-4 cells recognized by the delimitations of syncitia formation and higher ATP signal rather than bare carbon dots. The modified carbon dots also promote dual-action on HIV-1 treatment by both intracellularly and extracellularly viral blocking by combining with the Duviral drug, along with compressing p24 antigen signals that are better than APBA-CDs and Duviral itself.