Histopathological changes in the intestine of Channa micropeltes infected with the cestode Senga rostellarae

• Main Authors: Marina Hassan, ,-, Muhammad Syafiq Izzuddin Abdul Hadi, ,-, Mohd Fazrul HisamAbd Aziz, ,-, Wahidah Wahab, ,-, Farizan Abdullah, ,-, Shuhaimi Deraman, ,-, Kismiyati, ,-, Mohd Ihwan Zakariah, ,-
• Format: Article PeerReviewed
• Language: English; English; English; English
• Published: IOP Publishing Ltd.
• Subjects: SH1-691 Aquaculture. Fisheries. Angling; SH20.3-191 Aquaculture
• Online Access: https://repository.unair.ac.id/126743/1/IOP%20370%202019.pdf; https://repository.unair.ac.id/126743/2/Turnitin-Histopathological%20changes%20in%20the%20intestine%20of%20Channa%20micropeltes%20infected%20with%20the%20cestode%20Senga%20rostellarae.pdf; https://repository.unair.ac.id/126743/5/ethical%20clearence.pdf; https://repository.unair.ac.id/126743/6/form%2015%20IOP%20370-digabungkan.pdf; https://repository.unair.ac.id/126743/; https://iopscience.iop.org/article/10.1088/1755-1315/370/1/012055
• Institution: Universitas Airlangga

Channa micropeltes or toman is a native freshwater fish species and not commercially species in Malaysia. The fish become one of the anglers’ attractions to Kenyir Lake, especially for recreational fishing purpose. However, parasitic diseases are one of the most common problems for this fish population. Studies on parasites of C. micropeltes is essential to our knowledge for the maintenance of natural resources. So, the objectives of this study were to identify the cestode and observe the histopathological changes on gastrointestinal tract of infected C. micropeltes. About 15 C. micropeltes were caught by using fishing rod. The fish were pithing, dissected and gastrointestinal tract was removed, then placed on petri dish. The stomach and intestine were fixed in 10% buffered formalin and proceed for preparing histology slides by using standard method for histology. Then, the tissues were stained by using hematoxylin and eosin (H & E) stain. The tissue changes were described. The cestodes were collected, fixed in 70% ethanol, stained with aceto-carmine and prepared the permanent slide. The cestodes morphology were observed under microscope for identification. In this study, only Senga rostellarae was identified. The histopathological changes showed the intestine with severe villus damage with destruction of villi epithelium and necrosis. Some areas with cross section of cestode showed increase of goblet cells and generated necrosis. Based on this study, S. rostellarae was a dominant species, and its activities caused severe damage to the fish intestine. The conditions can cause death to the fish due to hemorrhage and malabsorption of nutrien