ACTH4-10PRO8-GLY9-PRO10 improves Neutrophil profile in spinal cord injury of rat models

• Main Authors: Muhammad Faris, Muhammad, Budi Utomo, Budi, Asra Al Fauzi, Asra, I. Ketut Sudiana, I. Ketut, Sri Maliawan, Sri, Yoes Prijatna Dahlan, Yoes, Oski Illiandri, Oski, Moh Hasan Machfoed, -, Abdul Hafid Bajamal, Abdul
• Format: Article PeerReviewed
• Language: Indonesian; Indonesian; English; English
• Published: Published bySociety of Pharmaceutical Education & Research [SPER
• Subjects: R5-920 Medicine (General)
• Online Access: https://repository.unair.ac.id/126901/1/4%20Artikel.pdf; https://repository.unair.ac.id/126901/2/04-%20Karil%20Review%201.pdf; https://repository.unair.ac.id/126901/3/Turnitin%204.pdf; https://repository.unair.ac.id/126901/4/Correspondence%204.pdf; https://repository.unair.ac.id/126901/; https://scholar.unair.ac.id/en/publications/acthsub4-10subprosup8sup-glysup9sup-prosup10sup-improves-neutroph; https://doi.org/10.51847/LWGHW1YN1Q
• Institution: Universitas Airlangga

Abstract Acute spinal cord injury (SCI) is a health burden that affects daily function. Based on its prevalence, SCI becomes a major problem in the country. Inflammation is one of SCI property that stays as the main target for management in the last decade. ACTH4-10PRO8-GLY9-PRO1 (Met-Glu-His-Phe-Pro-Gly-Pro) has been known for its anti-inflammatory properties but remains unclear for SCI models. This experimental study was done on the SCI model of the Sprague-Dawley rat. Neutrophil was taken as an inflammatory marker from the spinal cord of the rat model on 3 and 6 hours of observation following injury. Statistical analysis was done using ANOVA comparison test with significance cut off of p < 0.05, CI 95%. Six samples from each control and treatment group (normal saline 3 and 6 hours; ACTH4-10PRO8-GLY9-PRO10 3 and 6 hours) were observed for immunoreacted values of neutrophil staining to assess inflammatory reactions in spinal cord injury models. Statistical analysis continued with the Kruskal-Wallis test and found significant differences (p < 0.001) between groups. Post-hoc tests were carried out using the Steel method. The most significant difference in Neutrophil levels was found in the ACTH4-10PRO8-GLY9-PRO10 6 hours group against normal saline 6 hours.