The Effect of ACTH(4-10)PRO8-GLY9-PRO10 Administration on the Expression of IL-6 and IL8 in Sprague Dawley Mice with Spinal Cord Injury

• Main Authors: Muhammad Azzam, -, Achmad Fahmi, -, Budi Utomo, -, Muhammad Faris, -, Muhammad Arifin Parenrengi, -, I Ketut Sudiana, -, Abdul Hafid Bajamal, -, Eko Agus Subagio, -
• Format: Article PeerReviewed
• Language: English; Indonesian; English
• Published: Georg Thieme Verlag 2022
• Subjects: R5-920 Medicine (General)
• Online Access: https://repository.unair.ac.id/126909/1/11%20Artikel.pdf; https://repository.unair.ac.id/126909/2/11-%20Karil%20Review%201.pdf; https://repository.unair.ac.id/126909/3/Turnitin%2011.pdf; https://repository.unair.ac.id/126909/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357503/; https://doi.org/10.1055%2Fs-0042-1744468
• Institution: Universitas Airlangga

Abstract Background Spinal cord injury (SCI) is a significant cause of morbidity since it results in the inflammation process which leads to necrosis or apoptosis. Inflammatory response to the tissue damage increases IL-6 and IL-8 levels. ACTH4 – 10Pro8-Gly9-Pro10 is a peptide community that has been shown to have a beneficial effect on minimizing the morbidity and increasing the recovery time. Methods This study is a true experimental laboratory research with a totally randomized method. The subjects were animal models with light and extreme compression of spinal cord, respectively. Results The administration of ACTH 4–10 in mild SCI in the 3-hour observation group did not show a significant difference in IL-6 expression compared with the 6-hour observation group. The administration of ACTH 4–10 in severe SCI showed a significantly lower expression level of IL-6 in the 3-hour observation group compared with the 6-hour one. The administration of ACTH 4–10 in severe SCI led to a significantly lower IL-8 expression in the 3-hour observation group compared with the 6-hour one. However, there was no significant difference in IL-8 expression in the group receiving ACTH 4–10 in 3 hours observation compared with that in 6 hours observation. Conclusion The administration of ACTH4–10Pro8-Gly9-Pro10 can reduce the expression of IL-6 and IL-8 at 3-hour and 6-hour observation after mild and severe SCI in animal models. Future research works are recommended.