Topical Instillation of Resveratrol Preconditioned Wharton’s Jelly Mesenchymal Stem Cell Secretome Preserves Ocular Surface in Experimental Model of Severe Dry Eye Disease

• Main Authors: Diandra Astaridewi, -, Evelyn Komaratih, Evelyn, Yuyun Rindiastuti, -, Yohanes Widodo Wirohadidjojo, -, Djoko Legowo, -, Ni Made Inten Lestari, -, Diandra Astari Dewi, -, I. Made Satya, -, Fedik A. Rantam, -, Cita R. S. Prakoeswa, -
• Format: Article PeerReviewed
• Language: English; Indonesian; English
• Published: Scientific Foundation SPIROSKI
• Subjects: R5-920 Medicine (General)
• Online Access: https://repository.unair.ac.id/127049/1/05.%20Topical%20Instillation.pdf; https://repository.unair.ac.id/127049/2/05.pdf; https://repository.unair.ac.id/127049/3/05.%20Topical%20Instillation%20of%20Resveratrol.pdf; https://repository.unair.ac.id/127049/; https://oamjms.eu/index.php/mjms/article/view/5305; https://doi.org/10.3889/oamjms.2020.5305
• Institution: Universitas Airlangga

Abstract BACKGROUND: Secretome derived from Wharton’s jelly mesenchymal stem cells (WJ-MSC) has a beneficial effect for ocular surface regeneration; however, the high amount of vascular endothelial growth factor (VEGF) remains a challenge for its application. AIM: The aim of the study was to investigate the effect of resveratrol (RV) (VEGF reducing agent) preconditioned WJ-MSC secretome in Concanavalin A-induced severe dry eye model. METHODS: Pre- and post-experimental study composed of topical instillation of WJ-MSC secretome group, balanced salt solution control group, and normal control group. Tear production, tear break-up time (TBUT), corneal fluorescein dye staining, VEGF level in aqueous tear, goblet cell density, and inflammatory cells in the ocular surface were analyzed. RESULTS: Topical instillation of RV preconditioned WJ-MSC secretome successfully improved tear film production (p = 0.008) and TBUT (p = 0.008), promoted goblet cell restoration (p = 0.023) and reduced corneal fluorescein staining (p = 0.003), while inhibited infiltration of inflammatory cells (p = 0.01) and secretion of VEGF in aqueous tear (p = 0.003). CONCLUSION: Topical instillation of RV preconditioned WJ-MSC secretome has great potential as cell-free based therapy to preserve ocular surface in an experimental model of severe Dry eye disease.