Comparison Correlation of miR-150, hsa-let-7e, and miR-146a and gene expression of IL-6, IL-8, IP-10, and MIP-1β during dengue virus infection

• Main Authors: Sri Masyeni, -, Kuntaman Kuntaman, -, Aryati, -
• Format: Article NonPeerReviewed
• Language: English; English; English
• Published: Nara Medical assocication 2021
• Subjects: RB Pathology; RB37-56.5 Clinical pathology. Laboratory technique
• Online Access: https://repository.unair.ac.id/127084/1/artiekl%2011.pdf; https://repository.unair.ac.id/127084/2/turnitin%2011.pdf; https://repository.unair.ac.id/127084/3/karil%2011.pdf; https://repository.unair.ac.id/127084/; https://narraj.org/main/article/view/31/10
• Institution: Universitas Airlangga

Growing evidence suggeststhat microRNAs (miRNAs) play a pivotal role in viral infection. The objective of this study was to assess the association between the expression of miR-150, hsa-let-7e, and miR-146a on cytokine expression during dengue infection. Dengue virus (DENV) strain SJN-006, a serotype 2 DENV strain of the Cosmopolitan genotype, isolated in Bali, Indonesia, was used to infect peripheral blood mononuclear cells(PBMCs) isolated from healthy individuals. The relative gene expressions of miR-150, hsa-let-7e, and miR-146a as well as the gene expression of cytokines (IL-6,IL-8, IP-10, and MIP-1β) were determined using quantitative real time -polymerase chain reaction (qRT-PCR) at6, 12 and 24hourspost infection (hpi). Correlations between the microRNAs and cytokines were analyzed by means of causality tests. Our data suggeststhat miR-150 and hsa-let-7e were significantly higher in infected-PBMCs after 12 hpi compared to the uninfected-PBMCs (p<0.05). The causality tests demonstrated that miR-150 and hsa-let-7e were negatively correlated with IL-8expression, meanwhile miR-146a was thecontrast. DENV infection was negatively and positively correlated with miR-150 and hsa-let-7e, respectively, after 24 hpi. In conclusion, our data demonstrates the vital role of miR-150, hsa-let-7e, and miR-146a in regulating IL-8expression with possible different pathways