Treadted Plasmodium berghei infected pregnant mice by – Andrographis paniculata tablet (AS201 – 01) decreasing the TLR -4 expression and apoptosis index of placental tissue

• Main Authors: Budi Prasetyo, Budi, Diantri Nari Ratih, -, Yustinasari, -, Hilkatul Ilmi, -, Lidya Tumewu, -, Aty Widyawaruyanti, -
• Format: Article PeerReviewed
• Language: English; Indonesian; English
• Published: MediPoeia
• Subjects: R5-920 Medicine (General)
• Online Access: https://repository.unair.ac.id/127252/1/7.%20Treated%20Plasmodium%20berghei%20infected%20%29%20decreasing%20the%20TLR-4%20expression%20and%20apoptosis%20index%20of%20placental%20tissue.pdf; https://repository.unair.ac.id/127252/2/7.pdf; https://repository.unair.ac.id/127252/3/7%20turnitin.pdf; https://repository.unair.ac.id/127252/; https://scholar.unair.ac.id/en/publications/treated-plasmodium-berghei-infected-pregnant-mice-by-andrographis; https://doi.org/10.7324/JAPS.2018.8415
• Institution: Universitas Airlangga

Abstract Objective: This study aimed to evaluate the effect of Andrographis paniculata tablet (AS201-01) in decreasing expression of Toll-Like Receptor-4 (TLR-4) and apoptosis index of placental tissue of Plasmodium berghei infected pregnant mice. Material and methods: A total of 24 pregnant mice were divided into 4 groups, G1 was uninfected pregnant mice group, G2 was untreated Plasmodium berghei infected pregnant mice, G3 was P. berghei infected pregnant mice treated with AS201-01 tablet and G4 was P. berghei infected pregnant mice treated with dihydropiperaquine-phosphate (DHP) tablets. All mice were sacrifice at day 15th of pregnancy and the placenta was collected. The TLR-4 expression and apoptosis index were evaluated using immunohistochemistry assay. Results: The TLR-4 expression and apoptosis index on G3 was lower than G2 and showed a statistically significant difference (P = 0.00). The TLR-4 expression and apoptosis index of placental tissue in P. berghei infected pregnant mice which treated with AS201-01 tablet were decreased compared to untreated group and DHP tablet treated group as well. Conclusion: AS201-01 tablet containing ethyl acetate fraction of A. paniculata could decrease TLR-4 expression and apoptosis index of placental tissue in P. berghei infected pregnant mice.