Cytotoxicity Test of Tithonia diversifolia Leaf Extract on Bone Marrow Mesenchymal Stem Cell (BMSC) of Rats Using MTT Assay Method

• Main Authors: Hani Plumeriastuti, -, Agesti Veva Kalista, -, Budiastuti, -, Mustofa Helmi Effendi, -
• Format: Article PeerReviewed
• Language: English; English; English
• Published: Medknow Publications 2020
• Subjects: SF405.5-407 Laboratory animals
• Online Access: https://repository.unair.ac.id/127356/3/Artikel_25_Hani_Plumeriastuti.pdf; https://repository.unair.ac.id/127356/1/Kesesuaian_25_Hani_Plumeriastuti.pdf; https://repository.unair.ac.id/127356/2/Similarity_25_Hani_Plumeriastuti.pdf; https://repository.unair.ac.id/127356/
• Institution: Universitas Airlangga

The aim of this study was to identify the cytotoxicity effect of Tithonia diversifolia leaf extract on bone marrow mesenchymal stem cell using MTTassay. The sample of this research using bone marrow mesenchymal stem cell of rats. The Tithonia diversifolia leaf extract divide into 4 concentration (0.5%, 0.25%, 0.125%, and 0.0625%) and given to bone marrow mesenchymal stem cell of rats in each well and incubated for 24 hours. The toxicity result was obtained using the MTT assay method and incubate for 4 hours. The optical density absorbency which indicates cell viability, calculated by ELISA reader with a wavelength of 595nm. Cytotoxicity parameters using LC50 analyzed by probit analysis. The extract of Tithonia diversifolia leaf with the concentration of 0.5%, 0.25%, and 0.125% found to kill cell up to 50% and only the concentration 0.0625 of Tithonia diversifolia leaf extract with the highest cell viability. The LC50 value of Tithonia diversifolia leaf extract on bone marrow mesenchymal of rat is 0.167%