Corticosteroid Effects and Administration Time Difference on Mice Model of Biliary Atresia

Abstract Corticosteroid therapy (steroid) has evolved into a commonly selected therapy after portoenterostomy therapy and is believed to improve clinical outcomes in biliary atresia (BA). This study aims to evaluate the effects of corticosteroid administration and its administration time on in...

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Main Authors: Bagus Setyoboedi, Bagus, Anang Endaryanto, -, Sjamsul Arief, -
Format: Article PeerReviewed
Language:English
Indonesian
English
English
Indonesian
Published: Ektodermal Displazi Grubu
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Online Access:https://repository.unair.ac.id/127758/1/02.%20Corticosteroid%20Effects.pdf
https://repository.unair.ac.id/127758/2/02%20karil.pdf
https://repository.unair.ac.id/127758/3/02.%20Corticosteroid%20Effects.pdf
https://repository.unair.ac.id/127758/4/02.%20CORTICOSTEROID%20EFFECTS.pdf
https://repository.unair.ac.id/127758/9/2%20etik.pdf
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spelling id-langga.1277582023-10-17T04:17:45Z https://repository.unair.ac.id/127758/ Corticosteroid Effects and Administration Time Difference on Mice Model of Biliary Atresia Bagus Setyoboedi, Bagus Anang Endaryanto, - Sjamsul Arief, - R5-920 Medicine (General) Abstract Corticosteroid therapy (steroid) has evolved into a commonly selected therapy after portoenterostomy therapy and is believed to improve clinical outcomes in biliary atresia (BA). This study aims to evaluate the effects of corticosteroid administration and its administration time on innate immune response, the changes from innate to non-self adaptive immunity, non-self adaptive immunity, and self-adaptive immunity, as well as a biliary obstruction on animal models of biliary atresia. This study utilized a randomized multiple factorial and posttest-only control group designs on newborn Balb/c mice as the animal models. Forty-four mice were randomly categorized into two groups, i.e., the experimental and control groups. All mice underwent the examination of several liver variables, liver histopathology, as well as bile ducts after the termination process. Univariate Ttest and factorial MANOVA were employed for the data analysis. If the data were not normally distributed, the analysis could be carried out using the median and interquartile range, MannWhitney U test, and Kruskal-Wallis test. Dexamethasone administration, Rhesus Rotavirus (RRV) induction, and pain duration after RRV exposure provided interaction effects towards CD68 expression with the significance values as follows: day 7= (0.01), day 14 = (0.001), and day 21 = (0.035); CD39 expression on day 7 (0.01), day 14 (0.001), and day 21 (0.001); CD4 expression on day 7 (0.001), day 14 (0.018), and day 21 (0.018); CD8 expression on day 7 (0.001), day 14 (0.018), and day 21 (0.014); B cell expression on day 7 (0.001), day 14 (0.002), and day 21 (0.018); ANCA expression on day 7 (0.012), day 14 (0.05), and day 21 (0.001). After RRV induction, the immune response on animal models of biliary atresia increases. Dexamethasone administration on day 7 after RRV induction provides the most effective effects on immune response decrease. Ektodermal Displazi Grubu Article PeerReviewed text en https://repository.unair.ac.id/127758/1/02.%20Corticosteroid%20Effects.pdf text id https://repository.unair.ac.id/127758/2/02%20karil.pdf text en https://repository.unair.ac.id/127758/3/02.%20Corticosteroid%20Effects.pdf text en https://repository.unair.ac.id/127758/4/02.%20CORTICOSTEROID%20EFFECTS.pdf text id https://repository.unair.ac.id/127758/9/2%20etik.pdf Bagus Setyoboedi, Bagus and Anang Endaryanto, - and Sjamsul Arief, - Corticosteroid Effects and Administration Time Difference on Mice Model of Biliary Atresia. Journal of International Dental and Medical Research, 13 (2). ISSN 1309-100X https://www.jidmr.com/journal/wp-content/uploads/2020/06/28-FTM20_1143_Bagus_Setyoboedi_Indonesia.pdf
institution Universitas Airlangga
building Universitas Airlangga Library
continent Asia
country Indonesia
Indonesia
content_provider Universitas Airlangga Library
collection UNAIR Repository
language English
Indonesian
English
English
Indonesian
topic R5-920 Medicine (General)
spellingShingle R5-920 Medicine (General)
Bagus Setyoboedi, Bagus
Anang Endaryanto, -
Sjamsul Arief, -
Corticosteroid Effects and Administration Time Difference on Mice Model of Biliary Atresia
description Abstract Corticosteroid therapy (steroid) has evolved into a commonly selected therapy after portoenterostomy therapy and is believed to improve clinical outcomes in biliary atresia (BA). This study aims to evaluate the effects of corticosteroid administration and its administration time on innate immune response, the changes from innate to non-self adaptive immunity, non-self adaptive immunity, and self-adaptive immunity, as well as a biliary obstruction on animal models of biliary atresia. This study utilized a randomized multiple factorial and posttest-only control group designs on newborn Balb/c mice as the animal models. Forty-four mice were randomly categorized into two groups, i.e., the experimental and control groups. All mice underwent the examination of several liver variables, liver histopathology, as well as bile ducts after the termination process. Univariate Ttest and factorial MANOVA were employed for the data analysis. If the data were not normally distributed, the analysis could be carried out using the median and interquartile range, MannWhitney U test, and Kruskal-Wallis test. Dexamethasone administration, Rhesus Rotavirus (RRV) induction, and pain duration after RRV exposure provided interaction effects towards CD68 expression with the significance values as follows: day 7= (0.01), day 14 = (0.001), and day 21 = (0.035); CD39 expression on day 7 (0.01), day 14 (0.001), and day 21 (0.001); CD4 expression on day 7 (0.001), day 14 (0.018), and day 21 (0.018); CD8 expression on day 7 (0.001), day 14 (0.018), and day 21 (0.014); B cell expression on day 7 (0.001), day 14 (0.002), and day 21 (0.018); ANCA expression on day 7 (0.012), day 14 (0.05), and day 21 (0.001). After RRV induction, the immune response on animal models of biliary atresia increases. Dexamethasone administration on day 7 after RRV induction provides the most effective effects on immune response decrease.
format Article
PeerReviewed
author Bagus Setyoboedi, Bagus
Anang Endaryanto, -
Sjamsul Arief, -
author_facet Bagus Setyoboedi, Bagus
Anang Endaryanto, -
Sjamsul Arief, -
author_sort Bagus Setyoboedi, Bagus
title Corticosteroid Effects and Administration Time Difference on Mice Model of Biliary Atresia
title_short Corticosteroid Effects and Administration Time Difference on Mice Model of Biliary Atresia
title_full Corticosteroid Effects and Administration Time Difference on Mice Model of Biliary Atresia
title_fullStr Corticosteroid Effects and Administration Time Difference on Mice Model of Biliary Atresia
title_full_unstemmed Corticosteroid Effects and Administration Time Difference on Mice Model of Biliary Atresia
title_sort corticosteroid effects and administration time difference on mice model of biliary atresia
publisher Ektodermal Displazi Grubu
url https://repository.unair.ac.id/127758/1/02.%20Corticosteroid%20Effects.pdf
https://repository.unair.ac.id/127758/2/02%20karil.pdf
https://repository.unair.ac.id/127758/3/02.%20Corticosteroid%20Effects.pdf
https://repository.unair.ac.id/127758/4/02.%20CORTICOSTEROID%20EFFECTS.pdf
https://repository.unair.ac.id/127758/9/2%20etik.pdf
https://repository.unair.ac.id/127758/
https://www.jidmr.com/journal/wp-content/uploads/2020/06/28-FTM20_1143_Bagus_Setyoboedi_Indonesia.pdf
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