The Relationship of The Amount of Caspase 3 Protein Expression and Macrophage Cells to Apoptosis of Squamous Epithel Cells in Ropivacaine Additional Area Around Operating Incisions (Laboratory Experimental Study on Wistar Strain Male White Mice)

• Main Authors: Dana Wandrianbaraseta, -, Prananda Surya Airlangga, -, Imam Susilo, Imam, Prihatma Kriswidyatomo, -, Hamzah, -, Mahmudah, -
• Format: Article PeerReviewed
• Language: English; Indonesian; English; Indonesian
• Published: Mediscript Ltd.
• Subjects: R5-920 Medicine (General)
• Online Access: https://repository.unair.ac.id/127934/1/01.%20artikel.pdf; https://repository.unair.ac.id/127934/2/01.%20karil.pdf; https://repository.unair.ac.id/127934/7/10%20turnitin.pdf; https://repository.unair.ac.id/127934/8/10%20etik.pdf; https://repository.unair.ac.id/127934/; https://hivnursing.net/index.php/hiv/article/view/2078
• Institution: Universitas Airlangga

Abstract Surgery is sure to cause injury and cause pain which can interfere with wound healing if not handled adequately. In the process of wound healing, macrophages have a major role both as pro-inflammatory and anti-inflammatory agents, as well as the role of executors of cell death in regulating tissue hemostasis. Ropivacaine as a local anesthetic is believed to be able to help the wound healing process, apart from being an analgesic. Aim and Objective: This research was aimed to prove the effect of Ropivacaine on macrophages and caspase-3 which play a role in the wound healing process.Materials and Methods: This study is a quasi experimental prospective analysis with a randomized post-test-only control group design with white male Wistar rats as subjects. The samples were 24 subjects who met the inclusion and exclusion criteria which were divided into four groups, namely the treatment group on day 3 and 7 with 0.2% ropivacaine infiltration in the incision wound and the control group with 0.9% NaCl infiltration on day 3 and 7. Excisional biopsy procedures were done on third and seventh day. Examination under microscopy to assess expression caspase-3 and the amount of macrophages. Then analyzed by SPSS. Caspase-3 expression and the number of macrophage cells were lower than the control group (p<0.05).Results and Conclusion: There was a significant difference in the expression of caspase-3 protein in the control group on the 3rd day with the 3rd day treatment (p=0.007), the 7th day control group with the 7th day treatment (p=0.008), and the 7th day treatment group 3rd day with 7th day treatment (p=0.026), and the number of macrophage cells in all study groups (p=0.0001). Therefore, ropivacaine infiltration had an effect on decreasing the expression of caspase-3 protein and the number of macrophage cells on the 3rd and 7th day.