The Role of Ellagic Acid on Inflammatory Protein NFKB1 and HSP70 with STITCH Prediction

• Main Authors: Debby Saputera, -, Intan Nirwana, -, Michael Josef Kridanto Kamadjaja, -, Meircurius Dwi Condro Surboyo, -
• Format: Article PeerReviewed
• Language: English; English; English
• Published: Chiang Mai University Journal of Natural Sciences 2021
• Subjects: RK Dentistry
• Online Access: https://repository.unair.ac.id/127941/1/artikel.pdf; https://repository.unair.ac.id/127941/2/turnitin.pdf; https://repository.unair.ac.id/127941/3/karil.pdf; https://repository.unair.ac.id/127941/
• Institution: Universitas Airlangga

The objective of this study was to determine the intervention mechanism of Ellagic Acid (ES) against inflammatory proteins NFKB1 and HSP70. Target Protein Analysis using SeaTarget (http://sea.bkslab.org). The compound is then analyzed for its target protein in the human body using the STITCH database (http://stitch.embl.de/). Then analyzed the interactions between proteins using the STRINGdb database (https://string-db.org/cgi/input.pl). In the analysis results with SEA, Ellagic Acid can target HSP70. Based on the results of interactions using STITCH, ellagic acid can target the Alpha Protein Kinase Catalytic Sub-unit (PRKCA). Protein Kinase Catalytic Sub-unit Alpha facilitates ellagic acid interactions with Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1) and Heat Shock 60kDa Protein 1 (HSPD1). Where NFKB1 is a pleiotropic transcription factor in various processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. Ellagic acid can help reduce inflammation. It is predicted that the active compound in ellagic acid acts as an anti-inflammatory. The NFKB1 and HSP70 pathways play a role in inflammation and have activity for inflammation regulation.