Human Umbilical Cord Blood-derived Secretome Enhance Endothelial Progenitor Cells Migration on Hyperglycemic Conditions

• Main Authors: Yudi Her Oktaviono, -, Melly Susanti, -, Achmad Lefi, Achmad, Ferry Sandra, -
• Format: Article PeerReviewed
• Language: English; Indonesian; English
• Published: Pharmacognosy Network Worldwide 2020
• Subjects: R5-920 Medicine (General)
• Online Access: https://repository.unair.ac.id/128142/1/5.%20Human%20Umbilical%20Cord.pdf; https://repository.unair.ac.id/128142/2/5.pdf; https://repository.unair.ac.id/128142/3/5.%20Human%20Umbilical%20Cord%20Blood-derived%20Secretome%20Enhance.pdf; https://repository.unair.ac.id/128142/; https://mail.phcogj.com/article/1178; http://dx.doi.org/10.5530/pj.2020.12.113
• Institution: Universitas Airlangga

Abstract: Hyperglycemia state is harmful to body’s homeostasis. Uncontrolled hyperglycemic patients, especially patients with diabetes mellitus have a higher mortality risk of heart disease 2 to 4 times compared to non-hyperglycemic patients. Vascular endothelial impairment always been observed and found as a key feature of hyperglycemia state, which is correlated with reduced numbers and dysfunction of endothelial progenitor cells (EPCs). Objective: This paper aims to investigate the effect of hUCB-MSCs derived secretome treatment on the EPCs migration under hyperglycemia state. Materials and Methods: EPCs were isolated and cultured from peripheral blood samples and cultured for three days. Cultured EPCs were cultivated in 6-well plates until confluence and incubated with high glucose for 5 days, then placed in the modified Boyden chamber at the upper chamber with basal media. The lower chamber was supplemented with basal media and secretome at 2%, 10%, and 20% concentration and VEGF treated group as a control. EPCS migration was evaluated using a Boyden chamber assay. Statistical analysis was performed using SPS 25.0. Results: EPCs migration were significantly higher when hUCB-MSCs-derived secretome was given in high glucose concentrations compared to the and control group (79.80 ± 5.07 vs 51.00 ± 5.15, p<0.000). This study also showed that hUCB-MSCs-derived secretome increase EPCs migration under high glucose concentrations in a dose-dependent manner (p<0.05). Conclusion: hUCB-MSCsderived secretome enhances EPCs migration under hyperglycemic state. This result may be of relevance for cell-free and regenerative therapeutic modality for a diabetic patient with coronary artery disease (CAD)