Effect of ACTH4-10Pro8-Gly9-Pro10 on anti-inflammatory cytokine (IL-4, IL-10, IL-13) expression in acute spinal cord injury models (male Sprague Dawley rats)

• Main Author: Eko Agus Subagio, Eko
• Format: Article PeerReviewed
• Language: English; English; English; English
• Published: Publisher:Taylor & Francis 2023
• Subjects: R5-920 Medicine (General); RD92-97.8 Emergency surgery. Wounds and injuries
• Online Access: https://repository.unair.ac.id/128246/1/8f0ec09e-e9e1-4acf-a873-5193b963b231_127413_-_asadullah_asadullah.pdf; https://repository.unair.ac.id/128246/2/10%20-%20Effect%20of%20ACTH4-10Pro8-Gly9-Pro10%20on%20anti-inflammatory%20cytokine%20%28IL-4%2C%20IL-10%2C%20IL-13%29%20expression%20in%20acute%20spinal%20cord%20injury%20models%20%28male%20Sprague%20Dawley%20rats%29.pdf; https://repository.unair.ac.id/128246/3/corresponding.pdf; https://repository.unair.ac.id/128246/4/10%20-%20Effect%20of%20ACTH4-10Pro8-Gly9-Pro10%20on%20anti-inflammatory%20cytokine%20IL-4%2C%20IL-10%2C%20IL-13%20expression%20in%20acute%20spinal%20cord%20injury%20models%20-male%20Sprague%20Dawley%20rats.pdf; https://repository.unair.ac.id/128246/; https://doi.org/10.12688/f1000research.127413.1; https://doi.org/10.12688/f1000research.127413.1
• Institution: Universitas Airlangga

Background: Spinal cord injury (SCI) is a damage to the spinal cord caused mainly by trauma resulting in major motor, sensory and autonomic dysfunctions. Its final neurological outcome is determined by both primary and secondary injury processes. A key component of secondary injury mechanisms after initial trauma is neuroinflammation. A neuroprotective compound, ACTH4-10Pro8-Gly9- Pro10 (ACTH4-10) also known as semax, has shown neuroprotective and anti-inflammatory properties. ACTH4-10 has also been actively used in the treatment of brain ischemia without serious complication reported. Here, we analyzed the effects of ACTH4-10 at regulating the inflammatory cascade in SCI by looking at anti-inflammatory cytokine (IL-4, IL-10 and IL-13) levels after acute SCI. Method: We carried out laminectomies in male Sprague Dawley rats at the second thoracic vertebrae. After laminectomy, we exposed the myelum and created mild SCI models with 20-g, and severe SCI with 35-g aneurysm clips. ACTH4-10 was administered intranasally to the treatment group and 0.9% NaCl to the control group (placebo). Both groups were kept alive and terminated at 3 and 6 hours. The tissue sample preparations were fixed in formalin and examined for immunohistochemistry. Quantitative measurement of the cytokines was done in the posterior horn area with specific associated antimonoclonal antibodies. Results: Rats with mild SCI that were given ACTH4-10 showed greater anti-inflammatory levels at 3 hours post-compression but only IL-10 and IL-13 were elevated significantly at 6 hours. Rats with severe