Karakterisasi Formasi Biofilm In Vitro Streptococcus Uberis Penyebab Mastitis Kronis pada Sapi Perah
Mastitis is an inflammation of the mammary gland and is extremely problematic in the dairy industry. Streptococcus uberis is one of the main infectious agents responsible for causing mastitis. We speculate that one of the reasons behind the prevalence of S. uberis infections is its ability to for...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2012
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| Subjects: | |
| Online Access: | https://repository.unair.ac.id/129115/1/KKC%20KK%20TKH%20Vin%20k_ABSTRAK.pdf https://repository.unair.ac.id/129115/2/KKC%20KK%20TKH%20Vin%20k.pdf https://repository.unair.ac.id/129115/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | Mastitis is an inflammation of the mammary gland and is extremely
problematic in the dairy industry. Streptococcus uberis is one of the main
infectious agents responsible for causing mastitis. We speculate that one of the
reasons behind the prevalence of S. uberis infections is its ability to form biofilms.
The objectives of this study were to determine in vitro slime production, biofilm
formation and the presence of eX1:racelluler matrix associated with biofilm
production, we also observed the effect of casein, lactoferrin and skim milk in the
biofilm production. Twenty four strains of S. uberis isolated from the chronic
mastitic cows and \V'ere tested by microtiter plate (MP), Congo Red Agar (CRA)
and Scanning Electron Microscopy (SEM). The MP methods were used to
observed the effect of casein, lactoferrin and skim milk in the biofilm production.
Staphylococclls epidermidis ATCC 12228, was used as a negative control
Staphylococcus aurells ATCC 25923 and Staphylococcus simlllans ATCC 27851
was used as positive control. Of the 24 S. uberis strains were show positive result
in slime production using CRA method. All 24 strain also displayed a biofilmphenotype
when tested by MP. The strains were further classified as weak (56 %)
and strong (44 %) biofilm formers. Casein and skim milk 100 % can induce
bioflm production of S. lIberis, while lactoferrin did not. In summary, S. lIberis is
capable of forming biofilms in vitro on an abiotic surface. The economic impact
of Understanding the virulence factors that influence S. lIberis s ability to colonize
and maintain infections will ultimately allow for effective and appropriate
treatment protocols that could greatly decrease the impact of mastitis caused by
this pathogen in the dairy industry. |
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