IDENTIFIKASI GDF-9 (GROWTH DIFFERENTIATION FACTOR-9) PADA OOSIT KUMULUS KOMPLEKS SAPI YANG DIKOLEKSI DARI FOLIKEL ANTBRAL YANG TIDAK DIMATURASI SECARA IN VITRO
"Growth Differentiation Factor-9 (GDF-9) was glycoprotein as a part of Transforming Growth Factor Beta (TGFP) subfamily was secreted trough 51 kDa molecular weight oocyte. The purpose of the study was observed GDF .. 9 into oocytes cumulus complex that were collected from bovine anthral foll...
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Main Author: | |
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Format: | Theses and Dissertations NonPeerReviewed |
Language: | English |
Published: |
2010
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Subjects: | |
Online Access: | https://repository.unair.ac.id/130442/1/11.%20060610128.pdf https://repository.unair.ac.id/130442/ http://www.lib.unair.ac.id |
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Institution: | Universitas Airlangga |
Language: | English |
Summary: | "Growth Differentiation Factor-9 (GDF-9) was glycoprotein as a part of
Transforming Growth Factor Beta (TGFP) subfamily was secreted trough 51
kDa molecular weight oocyte. The purpose of the study was observed GDF .. 9
into oocytes cumulus complex that were collected from bovine anthral follicle.
Method of this study based on the specificity immunocytochemistry method
which avidin and biotin molecules boundary into the secondary antibody
avidin-.biotin complex shape. Total ammount of collected oocytes were 66
(100%) and total ammount of observerd oocytes were 47 (71,21 %) positive and
19 (28,79%) negative. Positive was marked by dark brown as an expression of
chromogen on the SA .. HRP complex boundaries. Negative was marked by
methylen green counterstain. However, GDP-9 was observed into the collected
oocytes from bovine anthral follicles immaturation in vitro process." |
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