Diagnostic Values of SARS-COV-2 Antibodies using Lifotronic ECL-8000
One of the most definitive diagnostic tests for COVID-19 infection is rRT-PCR. Another modality developed to diagnose COVID-19 infection is the antibody (serological) assay. This essay can be performed quickly and easily but requires high sensitivity and specificity. This study aims to analyze the d...
Saved in:
Main Authors: | , , |
---|---|
Format: | Article PeerReviewed |
Language: | English English English English |
Published: |
www.rjptonline.org
2023
|
Subjects: | |
Online Access: | https://repository.unair.ac.id/133334/2/Diagnostic%20values%20of%20SARS-CoV-2%20Antibodies%20using.pdf https://repository.unair.ac.id/133334/7/9_RJPT_16_11_2023%20%281%29%20%281%29-1.pdf https://repository.unair.ac.id/133334/1/T-Diagnostic%20Values%20of%20SARS-COV-2%20Antibodies%20using%20Lifotronic%20ECL-8000.pdf https://repository.unair.ac.id/133334/3/Diagnostic%20Values%20of%20SARS-COV-2%20Antibodies%20using.pdf https://repository.unair.ac.id/133334/ |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universitas Airlangga |
Language: | English English English English |
id |
id-langga.133334 |
---|---|
record_format |
dspace |
spelling |
id-langga.1333342024-07-05T09:08:52Z https://repository.unair.ac.id/133334/ Diagnostic Values of SARS-COV-2 Antibodies using Lifotronic ECL-8000 Ihda Kartika Syamsuddin Puspa Wardhani, - Aryati, - RB37-56.5 Clinical pathology. Laboratory technique One of the most definitive diagnostic tests for COVID-19 infection is rRT-PCR. Another modality developed to diagnose COVID-19 infection is the antibody (serological) assay. This essay can be performed quickly and easily but requires high sensitivity and specificity. This study aims to analyze the diagnostic values of anti-SARS-CoV-2 IgM and IgG using Lifotronic ECL-8000 and the development of antibodies based on time after the onset of the symptoms of COVID-19 in patients with confirmed COVID-19 infection. The serum of the patients with confirmed COVID-19 infection by rRT-PCR was collected between day 1 and day 21 after the onset of the symptoms. Anti-SARS-CoV-2 IgM and IgG from each sample were measured using Lifotronic ECL-8000 to determine their sensitivity, specificity, PPV, and NPV. This assay detects IgM against SARS-CoV-2 N and SRBD proteins, as well as IgG against SARS-CoV-2 SRBD proteins. The anti-SARS-CoV-2 IgM serological assays using Lifotronic ECL-8000 indicated that IgM had 91.6% sensitivity, 87.03% specificity, 90.4% PPV, and 88.67% NPV. Meanwhile, the anti-SARS-CoV-2 IgG serological assays using Lifotronic ECL-8000 showed that IgG had 93.05% sensitivity, 88.88% specificity, 91.78% PPV, and 90.56% NPV. The development of antibodies was observed on days 0-7 after the onset of the symptoms, and the positivity rate of anti-SARS-CoV-2 IgM was higher than that of anti-SARS-CoV-2 IgG. Starting from day 8 after the onset of the symptoms, the positivity rate of anti-SARS-CoV-2 IgG increased and remained higher than that of anti-SARS-CoV-2 IgM. It was concluded that anti-SARS-CoV-2 IgM and IgG serological assays using Lifotronic ECL-8000 could be utilized to support the diagnosis of patients with suspected COVID-19 infection with high sensitivity and specificity. www.rjptonline.org 2023 Article PeerReviewed text en https://repository.unair.ac.id/133334/2/Diagnostic%20values%20of%20SARS-CoV-2%20Antibodies%20using.pdf text en https://repository.unair.ac.id/133334/7/9_RJPT_16_11_2023%20%281%29%20%281%29-1.pdf text en https://repository.unair.ac.id/133334/1/T-Diagnostic%20Values%20of%20SARS-COV-2%20Antibodies%20using%20Lifotronic%20ECL-8000.pdf text en https://repository.unair.ac.id/133334/3/Diagnostic%20Values%20of%20SARS-COV-2%20Antibodies%20using.pdf Ihda Kartika Syamsuddin and Puspa Wardhani, - and Aryati, - (2023) Diagnostic Values of SARS-COV-2 Antibodies using Lifotronic ECL-8000. Research J. Pharm. and Tech, 16 (11). pp. 5034-5038. ISSN 0974-360X DOI: 10.52711/0974-360X.2023.00815 |
institution |
Universitas Airlangga |
building |
Universitas Airlangga Library |
continent |
Asia |
country |
Indonesia Indonesia |
content_provider |
Universitas Airlangga Library |
collection |
UNAIR Repository |
language |
English English English English |
topic |
RB37-56.5 Clinical pathology. Laboratory technique |
spellingShingle |
RB37-56.5 Clinical pathology. Laboratory technique Ihda Kartika Syamsuddin Puspa Wardhani, - Aryati, - Diagnostic Values of SARS-COV-2 Antibodies using Lifotronic ECL-8000 |
description |
One of the most definitive diagnostic tests for COVID-19 infection is rRT-PCR. Another modality developed to diagnose COVID-19 infection is the antibody (serological) assay. This essay can be performed quickly and easily but requires high sensitivity and specificity. This study aims to analyze the diagnostic values of anti-SARS-CoV-2 IgM and IgG using Lifotronic ECL-8000 and the development of antibodies based on time after the onset of the symptoms of COVID-19 in patients with confirmed COVID-19 infection. The serum of the patients with confirmed COVID-19 infection by rRT-PCR was collected between day 1 and day 21 after the onset of the symptoms. Anti-SARS-CoV-2 IgM and IgG from each sample were measured using Lifotronic ECL-8000 to determine their sensitivity, specificity, PPV, and NPV. This assay detects IgM against SARS-CoV-2 N and SRBD proteins, as well as IgG against SARS-CoV-2 SRBD proteins. The anti-SARS-CoV-2 IgM serological assays using Lifotronic ECL-8000 indicated that IgM had 91.6% sensitivity, 87.03% specificity, 90.4% PPV, and 88.67% NPV. Meanwhile, the anti-SARS-CoV-2 IgG serological assays using Lifotronic ECL-8000 showed that IgG had 93.05% sensitivity, 88.88% specificity, 91.78% PPV, and 90.56% NPV. The development of antibodies was observed on days 0-7 after the onset of the symptoms, and the positivity rate of anti-SARS-CoV-2 IgM was higher than that of anti-SARS-CoV-2 IgG. Starting from day 8 after the onset of the symptoms, the positivity rate of anti-SARS-CoV-2 IgG increased and remained higher than that of anti-SARS-CoV-2 IgM. It was concluded that anti-SARS-CoV-2 IgM and IgG serological assays using Lifotronic ECL-8000 could be utilized to support the diagnosis of patients with suspected COVID-19 infection with high sensitivity and specificity. |
format |
Article PeerReviewed |
author |
Ihda Kartika Syamsuddin Puspa Wardhani, - Aryati, - |
author_facet |
Ihda Kartika Syamsuddin Puspa Wardhani, - Aryati, - |
author_sort |
Ihda Kartika Syamsuddin |
title |
Diagnostic Values of SARS-COV-2 Antibodies using Lifotronic ECL-8000 |
title_short |
Diagnostic Values of SARS-COV-2 Antibodies using Lifotronic ECL-8000 |
title_full |
Diagnostic Values of SARS-COV-2 Antibodies using Lifotronic ECL-8000 |
title_fullStr |
Diagnostic Values of SARS-COV-2 Antibodies using Lifotronic ECL-8000 |
title_full_unstemmed |
Diagnostic Values of SARS-COV-2 Antibodies using Lifotronic ECL-8000 |
title_sort |
diagnostic values of sars-cov-2 antibodies using lifotronic ecl-8000 |
publisher |
www.rjptonline.org |
publishDate |
2023 |
url |
https://repository.unair.ac.id/133334/2/Diagnostic%20values%20of%20SARS-CoV-2%20Antibodies%20using.pdf https://repository.unair.ac.id/133334/7/9_RJPT_16_11_2023%20%281%29%20%281%29-1.pdf https://repository.unair.ac.id/133334/1/T-Diagnostic%20Values%20of%20SARS-COV-2%20Antibodies%20using%20Lifotronic%20ECL-8000.pdf https://repository.unair.ac.id/133334/3/Diagnostic%20Values%20of%20SARS-COV-2%20Antibodies%20using.pdf https://repository.unair.ac.id/133334/ |
_version_ |
1806060395821006848 |