AKTIVITAS ANTIOKSIDAN DARI EKSTRAK BUAH MENGKUDU ( Morinda citrifolia Linn ) PADA TIKUS DEWASA YANG DIINDUKSI ALLOXAN : PENELITTAN EKSPERIMENTAL LABORATORIS

• Main Author: PADOLI, 090014152M
• Format: Theses and Dissertations NonPeerReviewed
• Language: Indonesian; Indonesian
• Published: 2003
• Subjects: K85-89 Legal research; RB37-56.5 Clinical pathology. Laboratory technique; SF405.5-407 Laboratory animals
• Online Access: http://repository.unair.ac.id/36125/1/gdlhub-gdl-s2-2006-padoli-1919-tkd040-k.pdf; http://repository.unair.ac.id/36125/13/gdlhub-gdl-s2-2006-padoli-1919-tkd0406.pdf; http://repository.unair.ac.id/36125/; http://lib.unair.ac.id
• Institution: Universitas Airlangga

Type I diabetes is thought to occur as a result of loss insulin-producing pancreatic R cells by environmentally triggered increased free radicals. In rodent model of diabetic, alloxan, a genotoxic methylating agent that targeted to 13 cells and hepar tissue, is used to triggerthe initial death. The objective of study was to find out the influence of morinda citrifolia linn extract on the antioxidant effect against alloxan induced free radicals in rats. The rats were divided randomly into six group. Alloxan was dissolved in aquabidest and injected intraperitoneally, either as a single dose of 115 mg/kg. One week after alloxan treatment , the Morinda citrifolia linn extract was administered by nasogastric tubing at a dose of 1500 mg/kg of body weight and 2000 mg/kg of body weight for one weeks, and at a dose of 2000 mg/kg of body weight for two weeks. Positif control groups were similarly injected with vehicle only and negatif control group one (was not injected alloxan and neither administered morinda citrifolia linn). The results obtained with groups from each study were first analyzed using analysis of variance. Groups that showed different were further analyzed bay LSD test. The result of first study showed the morinda citrifolia extract of 2000 mg/Kg BW for one week or two weeks was successfully increased superoxide dismutase enzym, but not at 1500 mg/KgBW dose. The result of second study showed the morinda citrifolia extract of 2000 mg/Kg BW or 1500 mg/KgBW doses for one week was successfully decreased malonaldehyde enzym, but not at 2000 mg/KgBW dose for two weeks. The result of third study showed a significant different between the control and treatment groups with morinda citrifolia linn against alloxan induced in rats (p=0.0000). From the result suggest that the norinda citrifolia line extract has antioxidant effect which dose dependent manner.