IMUNOSENSOR UNTUK MENDETEKSI IMUNOGLOBULIN G TERHADAP Toxoplasma gondii DENGAN METODE GICA MENGGUNAKAN EKSTRAK ESA SEBAGAI BIORESEPTOR
Evaluation of detection of the IgG in human suggests that this test is rapid, simple, accurate, relatively inexpensive and suitable for use under field conditions. A gold immunochromatographic test using crude of Excretory and Secretory Antigens (ESAs) for detection of IgG against Toxoplasma gondii...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2010
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| Online Access: | http://repository.unair.ac.id/38350/2/gdlhub-gdl-s3-2011-dewilalebu-14484-tk0210-k.pdf http://repository.unair.ac.id/38350/1/gdlhub-gdl-s3-2011-dewilalebu-12158-tk0210.pdf http://repository.unair.ac.id/38350/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | Evaluation of detection of the IgG in human suggests that this test is rapid, simple, accurate, relatively inexpensive and suitable for use under field conditions. A gold immunochromatographic test using crude of Excretory and Secretory Antigens (ESAs) for detection of IgG against Toxoplasma gondii was developed. The study was a laboratory experimental. Data were analyzed statistically using Chi-Square with significance level of more than 0,05 (> 0,05). Forty nine collected samples from Laboratorium Kedung Doro Surabaya were analyzed by GICA, and the result was compared with ELISA. Analysis of the samples by ELISA indicated that thirty-two serum sample were positive and seventeen serum sample were negative Mean while, analysis of the samples by GICA showed that thirty- five serum sample were positive and fourteen serum sample were negative that examined by GICA. The relative sensitivity and spesificity of GICA were 100% and 82,4% when ELISA was used as reference. Statistical analysis by Chi-Square showed significant differences between ELISA and GICA. |
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