IMUNOGENISITAS DAN EFIKASI PROTEKTIF VAKSIN SUBUNIT SLPS DAN VAKSIN STRAIN RB51 PADA MENCIT (Mus musculus) TERHADAP INFEKSI B. abortus ISOLAT LAPANG PENELITIAN EKSPERIMENTAL LABORATORIS

• Main Author: SAIFUL ANIS, 061414253002
• Format: Theses and Dissertations NonPeerReviewed
• Language: Indonesian; Indonesian
• Published: 2016
• Subjects: SF811-909 Veterinary medicine of special organs, regions, and systems
• Online Access: http://repository.unair.ac.id/53543/1/TPKMV.%2015-16%20Ani%20i%20Abstrak.pdf; http://repository.unair.ac.id/53543/2/TPKMV.%2015-16%20Ani%20i.pdf; http://repository.unair.ac.id/53543/; http://lib.unair.ac.id
• Institution: Universitas Airlangga

This study was conducted to evaluate the immunogenicity and protective efficacy of smooth Brucella abortus lipopolysaccharide (SLPS) as a subunit vaccine. Subcutaneous injection of SLPS with Al(OH)3 and montanide as adjuvant into BALB/c mice elicited both humoral and cellular immune responses. Animals injected with SLPS develop antibodies without statistical differences compared to animals vaccinated with B. Abortus vaccine SRB51, which exhibited a dominance of immunoglobulin G2b (IgG2b) over IgG3, IgG2a and IgG1. In addition, the SLPS subunit vaccine induce T-cell-proliferative response characterized by the production IL-2 and also induced the production of gamma interferon, suggesting the induction of a typical T-helper-1-dominated immune response in mice. The SLPS subunit vaccine induced a strong, significant level of protection in BALB/c mice against challenge with B. Abortus virulent; the protection unit was lower than the one induced by B. Abortus vaccine SRB51. Altogether, these data suggest that the SLPS subunit vaccine is a good candidate for use in future studies of vaccination against brucellosis. Key words: Brucella abortus, SLPS, subunit vaccine, immunoglobulin, protection unit.