Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells(hUCMSCs) with Gelatin Solvent

• Main Authors: Nike Hendrijantini, NIDN. 0006105908, Utari Kresnoadi, NIDN. 0011015403, Sherman Salim, NIDN. 8833370018, Bambang Agustono, NIDN. 0027086304, Endang Retnowati, NIDN. 071608640, Iwan Syahrial, Pungky Mulawardhana, NIDN. 0003068107, Manggala Pasca Wardhana, NIDN. 0020118404, Coen Pramono, NIDN. 0010025405, Fedik Abdul Rantam, NIDN. 0010035907
• Format: Article PeerReviewed
• Language: English; Indonesian; Indonesian; English
• Published: Scientific Research Publishing 2015
• Subjects: R Medicine; RK Dentistry; RK641-667 Prosthetic dentistry. Prosthodontics
• Online Access: http://repository.unair.ac.id/54565/1/SHERMAN%2017.pdf; http://repository.unair.ac.id/54565/2/SHERMAN%2017%281%29.pdf; http://repository.unair.ac.id/54565/13/per%20nike%208.pdf; http://repository.unair.ac.id/54565/14/8.%20Study%20Biocompatibility%20and%20Osteogenic%20Differentiation%20Potential%20of%20Human%20Umbilical%20Cord%20Mesenchymal%20Stem%20Cells%20%28hUCMSCs%29%20with%20Gelatin%20Solvent.pdf; http://repository.unair.ac.id/54565/; http://file.scirp.org/pdf/JBiSE_2015070916344096.pdf; http://dx.doi.org/10.4236/jbise.2015.87039
• Institution: Universitas Airlangga

The human umbilical cord is a source of numerous Mesenchymal Stem Cells (MSCs), making it as a potential source of allogeneic multipotent cell for bone tissue engineering. The aims of this study were to find: 1) Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) phenotypic characterization, 2) The in-vitro osteogenic differentiation potential of hUCMSCs, 3) The cytotoxicity of gelatin solvent to hUCMSCs using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide(MTT) assay. As a result, through characterization of hUCMSCs, the majority of target cells expressed specific MSCs markers, Cellular Differentiation (CD)73, smaller number of subpopulation expressed CD90 with only minimal subpopulation expressed CD105 and all negative MSCs markers. Osteoblastic differentiation was found in a significantly high number of cells when in vitro osteogenic differentiation of hUCMSCs with Alizarin Red staining was done. Biocompatibility analysis using the MTT assay showed that gelatin solvent and Alpha modification of minimum essential