Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells(hUCMSCs) with Gelatin Solvent
The human umbilical cord is a source of numerous Mesenchymal Stem Cells (MSCs), making it as a potential source of allogeneic multipotent cell for bone tissue engineering. The aims of this study were to find: 1) Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) phenotypic characterization, 2) Th...
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2015
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Online Access: | http://repository.unair.ac.id/54565/1/SHERMAN%2017.pdf http://repository.unair.ac.id/54565/2/SHERMAN%2017%281%29.pdf http://repository.unair.ac.id/54565/13/per%20nike%208.pdf http://repository.unair.ac.id/54565/14/8.%20Study%20Biocompatibility%20and%20Osteogenic%20Differentiation%20Potential%20of%20Human%20Umbilical%20Cord%20Mesenchymal%20Stem%20Cells%20%28hUCMSCs%29%20with%20Gelatin%20Solvent.pdf http://repository.unair.ac.id/54565/ http://file.scirp.org/pdf/JBiSE_2015070916344096.pdf http://dx.doi.org/10.4236/jbise.2015.87039 |
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id-langga.545652019-09-23T02:47:26Z http://repository.unair.ac.id/54565/ Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells(hUCMSCs) with Gelatin Solvent Nike Hendrijantini, NIDN. 0006105908 Utari Kresnoadi, NIDN. 0011015403 Sherman Salim, NIDN. 8833370018 Bambang Agustono, NIDN. 0027086304 Endang Retnowati, NIDN. 071608640 Iwan Syahrial Pungky Mulawardhana, NIDN. 0003068107 Manggala Pasca Wardhana, NIDN. 0020118404 Coen Pramono, NIDN. 0010025405 Fedik Abdul Rantam, NIDN. 0010035907 R Medicine RK Dentistry RK641-667 Prosthetic dentistry. Prosthodontics The human umbilical cord is a source of numerous Mesenchymal Stem Cells (MSCs), making it as a potential source of allogeneic multipotent cell for bone tissue engineering. The aims of this study were to find: 1) Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) phenotypic characterization, 2) The in-vitro osteogenic differentiation potential of hUCMSCs, 3) The cytotoxicity of gelatin solvent to hUCMSCs using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide(MTT) assay. As a result, through characterization of hUCMSCs, the majority of target cells expressed specific MSCs markers, Cellular Differentiation (CD)73, smaller number of subpopulation expressed CD90 with only minimal subpopulation expressed CD105 and all negative MSCs markers. Osteoblastic differentiation was found in a significantly high number of cells when in vitro osteogenic differentiation of hUCMSCs with Alizarin Red staining was done. Biocompatibility analysis using the MTT assay showed that gelatin solvent and Alpha modification of minimum essential Scientific Research Publishing 2015 Article PeerReviewed text en http://repository.unair.ac.id/54565/1/SHERMAN%2017.pdf text id http://repository.unair.ac.id/54565/2/SHERMAN%2017%281%29.pdf text id http://repository.unair.ac.id/54565/13/per%20nike%208.pdf text en http://repository.unair.ac.id/54565/14/8.%20Study%20Biocompatibility%20and%20Osteogenic%20Differentiation%20Potential%20of%20Human%20Umbilical%20Cord%20Mesenchymal%20Stem%20Cells%20%28hUCMSCs%29%20with%20Gelatin%20Solvent.pdf Nike Hendrijantini, NIDN. 0006105908 and Utari Kresnoadi, NIDN. 0011015403 and Sherman Salim, NIDN. 8833370018 and Bambang Agustono, NIDN. 0027086304 and Endang Retnowati, NIDN. 071608640 and Iwan Syahrial and Pungky Mulawardhana, NIDN. 0003068107 and Manggala Pasca Wardhana, NIDN. 0020118404 and Coen Pramono, NIDN. 0010025405 and Fedik Abdul Rantam, NIDN. 0010035907 (2015) Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells(hUCMSCs) with Gelatin Solvent. Journal Biomedical Science and Engineering, 8 (7). pp. 420-428. ISSN 1937-6871 http://file.scirp.org/pdf/JBiSE_2015070916344096.pdf http://dx.doi.org/10.4236/jbise.2015.87039 |
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R Medicine RK Dentistry RK641-667 Prosthetic dentistry. Prosthodontics |
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R Medicine RK Dentistry RK641-667 Prosthetic dentistry. Prosthodontics Nike Hendrijantini, NIDN. 0006105908 Utari Kresnoadi, NIDN. 0011015403 Sherman Salim, NIDN. 8833370018 Bambang Agustono, NIDN. 0027086304 Endang Retnowati, NIDN. 071608640 Iwan Syahrial Pungky Mulawardhana, NIDN. 0003068107 Manggala Pasca Wardhana, NIDN. 0020118404 Coen Pramono, NIDN. 0010025405 Fedik Abdul Rantam, NIDN. 0010035907 Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells(hUCMSCs) with Gelatin Solvent |
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The human umbilical cord is a source of numerous Mesenchymal Stem Cells (MSCs), making it as a potential source of allogeneic multipotent cell for bone tissue engineering. The aims of this study were to find: 1) Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) phenotypic characterization, 2) The in-vitro osteogenic differentiation potential of hUCMSCs, 3) The cytotoxicity of gelatin solvent to hUCMSCs using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide(MTT) assay. As a result, through characterization of hUCMSCs, the majority of target cells expressed specific MSCs markers, Cellular Differentiation (CD)73, smaller number of subpopulation expressed CD90 with only minimal subpopulation expressed CD105 and all negative MSCs markers. Osteoblastic differentiation was found in a significantly high number of cells when in vitro osteogenic differentiation of hUCMSCs with Alizarin Red staining was done. Biocompatibility analysis using the MTT assay showed that gelatin solvent and Alpha modification of minimum essential |
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Article PeerReviewed |
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Nike Hendrijantini, NIDN. 0006105908 Utari Kresnoadi, NIDN. 0011015403 Sherman Salim, NIDN. 8833370018 Bambang Agustono, NIDN. 0027086304 Endang Retnowati, NIDN. 071608640 Iwan Syahrial Pungky Mulawardhana, NIDN. 0003068107 Manggala Pasca Wardhana, NIDN. 0020118404 Coen Pramono, NIDN. 0010025405 Fedik Abdul Rantam, NIDN. 0010035907 |
author_facet |
Nike Hendrijantini, NIDN. 0006105908 Utari Kresnoadi, NIDN. 0011015403 Sherman Salim, NIDN. 8833370018 Bambang Agustono, NIDN. 0027086304 Endang Retnowati, NIDN. 071608640 Iwan Syahrial Pungky Mulawardhana, NIDN. 0003068107 Manggala Pasca Wardhana, NIDN. 0020118404 Coen Pramono, NIDN. 0010025405 Fedik Abdul Rantam, NIDN. 0010035907 |
author_sort |
Nike Hendrijantini, NIDN. 0006105908 |
title |
Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells(hUCMSCs) with Gelatin Solvent |
title_short |
Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells(hUCMSCs) with Gelatin Solvent |
title_full |
Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells(hUCMSCs) with Gelatin Solvent |
title_fullStr |
Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells(hUCMSCs) with Gelatin Solvent |
title_full_unstemmed |
Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells(hUCMSCs) with Gelatin Solvent |
title_sort |
study biocompatibility and osteogenic differentiation potential of human umbilical cord mesenchymal stem cells(hucmscs) with gelatin solvent |
publisher |
Scientific Research Publishing |
publishDate |
2015 |
url |
http://repository.unair.ac.id/54565/1/SHERMAN%2017.pdf http://repository.unair.ac.id/54565/2/SHERMAN%2017%281%29.pdf http://repository.unair.ac.id/54565/13/per%20nike%208.pdf http://repository.unair.ac.id/54565/14/8.%20Study%20Biocompatibility%20and%20Osteogenic%20Differentiation%20Potential%20of%20Human%20Umbilical%20Cord%20Mesenchymal%20Stem%20Cells%20%28hUCMSCs%29%20with%20Gelatin%20Solvent.pdf http://repository.unair.ac.id/54565/ http://file.scirp.org/pdf/JBiSE_2015070916344096.pdf http://dx.doi.org/10.4236/jbise.2015.87039 |
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1681147090933121024 |