Suplementasi Protein Spesifik Membran Spermatozoa Dalam Media Diluter Semen Beku Sebagai Upaya Peningkatan Kualitas Semen Beku Sapi Post Thawing
This research aims to improve the quality of post-thawing of frozen semen, with the addition of specific protein osteopontin spermatozoa membrane into a freezing medium. This research was conducted in the laboratory and in the field that includes the stages following methods frozen semen quality tes...
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Online Access: | http://repository.unair.ac.id/54933/1/PEER%20REVIEW014.pdf http://repository.unair.ac.id/54933/16/20170316113122-min.pdf http://repository.unair.ac.id/54933/ http://journal.unair.ac.id/suplementation-of-specific-protein-membrane-spermatozoa-in-freezing-medium-to-improvement-efforts-a-succes-cattle-frozen-semen-quality-post-thawing-article-10277-media-28-category-5.html |
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id-langga.549332018-01-23T00:05:41Z http://repository.unair.ac.id/54933/ Suplementasi Protein Spesifik Membran Spermatozoa Dalam Media Diluter Semen Beku Sebagai Upaya Peningkatan Kualitas Semen Beku Sapi Post Thawing Hernawati, Tatik Mulyati, Sri Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology SF811-909 Veterinary medicine of special organs, regions, and systems This research aims to improve the quality of post-thawing of frozen semen, with the addition of specific protein osteopontin spermatozoa membrane into a freezing medium. This research was conducted in the laboratory and in the field that includes the stages following methods frozen semen quality test supplemented osteopontin spermatozoa membranes isolated cow. The test includes: measuring the levels of osteopontin using the standard osteopontin ELISA, determination of the optimum dosage of the addition of osteopontin in freezing media on the quality of spermatozoa post thawing, which include : the percentage of motility, viability, plasma membrane intact and acrosome intact hood. Results showed that the addition of specific protein osteopontin membrane of the spermatozoa in the semen freezing diluter media there are significant differences (p < 0.05) compared with no addition of osteopontin this means that the addition of specific protein osteopontin membrane can increase the number of spermatozoa motility, viability, plasma membrane intact (MPU). The conclusion from this study is the addition of a specific protein osteopontin spermatozoa membrane can improve the quality of frozen semen and improvement of in vitro fertilization. specific proteins, membrane spermatozoon, Diluter, Semen Quality FKH Unair 2015-11 Article PeerReviewed text id http://repository.unair.ac.id/54933/1/PEER%20REVIEW014.pdf text id http://repository.unair.ac.id/54933/16/20170316113122-min.pdf Hernawati, Tatik and Mulyati, Sri (2015) Suplementasi Protein Spesifik Membran Spermatozoa Dalam Media Diluter Semen Beku Sebagai Upaya Peningkatan Kualitas Semen Beku Sapi Post Thawing. Veterinaria Medika, 8 (3). pp. 289-300. ISSN 1979-1305 http://journal.unair.ac.id/suplementation-of-specific-protein-membrane-spermatozoa-in-freezing-medium-to-improvement-efforts-a-succes-cattle-frozen-semen-quality-post-thawing-article-10277-media-28-category-5.html |
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Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology SF811-909 Veterinary medicine of special organs, regions, and systems |
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Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology SF811-909 Veterinary medicine of special organs, regions, and systems Hernawati, Tatik Mulyati, Sri Suplementasi Protein Spesifik Membran Spermatozoa Dalam Media Diluter Semen Beku Sebagai Upaya Peningkatan Kualitas Semen Beku Sapi Post Thawing |
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This research aims to improve the quality of post-thawing of frozen semen, with the addition of specific protein osteopontin spermatozoa membrane into a freezing medium. This research was conducted in the laboratory and in the field that includes the stages following methods frozen semen quality test supplemented osteopontin spermatozoa membranes isolated cow. The test includes: measuring the levels of osteopontin using the standard osteopontin ELISA, determination of the optimum dosage of the addition of osteopontin in freezing media on the quality of spermatozoa post thawing, which include : the percentage of motility, viability, plasma membrane intact and acrosome intact hood. Results showed that the addition of specific protein osteopontin membrane of the spermatozoa in the semen freezing diluter media there are significant differences (p < 0.05) compared with no addition of osteopontin this means that the addition of specific protein osteopontin membrane can increase the number of spermatozoa motility, viability, plasma membrane intact (MPU). The conclusion from this study is the addition of a specific protein osteopontin spermatozoa membrane can improve the quality of frozen semen and improvement of in vitro fertilization.
specific proteins, membrane spermatozoon, Diluter, Semen Quality |
format |
Article PeerReviewed |
author |
Hernawati, Tatik Mulyati, Sri |
author_facet |
Hernawati, Tatik Mulyati, Sri |
author_sort |
Hernawati, Tatik |
title |
Suplementasi Protein Spesifik Membran Spermatozoa Dalam Media Diluter Semen Beku Sebagai Upaya Peningkatan Kualitas Semen Beku Sapi Post Thawing |
title_short |
Suplementasi Protein Spesifik Membran Spermatozoa Dalam Media Diluter Semen Beku Sebagai Upaya Peningkatan Kualitas Semen Beku Sapi Post Thawing |
title_full |
Suplementasi Protein Spesifik Membran Spermatozoa Dalam Media Diluter Semen Beku Sebagai Upaya Peningkatan Kualitas Semen Beku Sapi Post Thawing |
title_fullStr |
Suplementasi Protein Spesifik Membran Spermatozoa Dalam Media Diluter Semen Beku Sebagai Upaya Peningkatan Kualitas Semen Beku Sapi Post Thawing |
title_full_unstemmed |
Suplementasi Protein Spesifik Membran Spermatozoa Dalam Media Diluter Semen Beku Sebagai Upaya Peningkatan Kualitas Semen Beku Sapi Post Thawing |
title_sort |
suplementasi protein spesifik membran spermatozoa dalam media diluter semen beku sebagai upaya peningkatan kualitas semen beku sapi post thawing |
publisher |
FKH Unair |
publishDate |
2015 |
url |
http://repository.unair.ac.id/54933/1/PEER%20REVIEW014.pdf http://repository.unair.ac.id/54933/16/20170316113122-min.pdf http://repository.unair.ac.id/54933/ http://journal.unair.ac.id/suplementation-of-specific-protein-membrane-spermatozoa-in-freezing-medium-to-improvement-efforts-a-succes-cattle-frozen-semen-quality-post-thawing-article-10277-media-28-category-5.html |
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