Effects of Honey to mobilize endogenous stem cells in efforts intestinal and ovarian tissue regeneration in rats with protein energy malnutrition

• Main Authors: Prasetyo, R. Heru, Safitri, Erma
• Format: Article PeerReviewed
• Language: English; Indonesian
• Published: 2016
• Subjects: RC109-216 Infectious and parasitic diseases
• Online Access: http://repository.unair.ac.id/55106/2/Peerreview3.Effect%20of%20Honey.pdf; http://repository.unair.ac.id/55106/13/karil3.%20effect%20of%20honey%20oke-min.pdf; http://repository.unair.ac.id/55106/; http://ac.els-cdn.com/S2305050016300513/1-s2.0-S2305050016300513-main.pdf?_tid=08eb9a28-0ea7-11e7-b484-00000aab0f02&acdnat=1490149803_767038105beab35c3a9913423beafee4
• Institution: Universitas Airlangga

Objective: Auto-regeneration of the intestinal and ovarian tissue are experiencing degenerative due to protein energy malnutrition (PEM) through of auto-mobilization, increase of immune response and differentiation of endogenous stem cells. Methods: Female rat model of PEM obtained through fasting meals for 5 d and only to drink, causing malnutrition and damage of the intestinal and ovarian tissue. Furthermore, the therapy of honey with a dose of 30% water (T1) and 50% water (T2), respectively for 5 d and compared with the positive control, were fasted without being given honey (T0+) and negative control, not fasted and without being given honey (T0 − ). Observations on: auto-mobilization, increased immune response, differentiation of stem cells, and regen- eration of the intestinal and ovarian tissue with HE staining. Results: Auto-mobilization of stem cells based on the expression of CD34+ and CD45+, which is a marker of endogenous stem cells (hematopoietic stem cells/HSCs). Increased immune response is based on Hsp70 expression and PGE2 in intestinal tissue. Differ- entiation of stem cells into progenitor cells that expected based expression of growth differentiation factor-9 (GDF-9) by immunohistochemistry in ovarian tissue. Conclusions: Expression of CD34+ and CD45+, which signi fi cantly different in treat- ment 2 (2). Furthermore, increase of immune response (decrease Hsp70 expression and increased PGE2) in intestinal tissue. Increased immune response causes expression of GDF-9 in ovarian tissue. Decreased of Hsp70 expression, increased PGE2 and increased GDF-9 followed the process of regeneration of the intestinal and ovarian tissue.