IDENTIFIKASI FRAKSI AKTIF ANTIVIRUS HEPATITIS C DARI EKSTRAK ETANOL 80% HERBA Scoparia dulcis Linn.
Scoparia dulcis is a plant that belongs to Scrophulariaceae family. It used traditionally as remedies for stomach trouble. Previous study showed that ethanol extract 80% of Scoparia dulcis herbs has antiviral activity againts hepatitis C virus JFH1a infected hepatocyte cell Huh7it with IC50 valu...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/55652/1/FF.FT.%2021-16%20Sho%20i%20%20abstrak.pdf http://repository.unair.ac.id/55652/13/FF.FT.%2021-16%20Sho%20i-min.pdf http://repository.unair.ac.id/55652/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | Scoparia dulcis is a plant that belongs to Scrophulariaceae family. It
used traditionally as remedies for stomach trouble. Previous study showed
that ethanol extract 80% of Scoparia dulcis herbs has antiviral activity
againts hepatitis C virus JFH1a infected hepatocyte cell Huh7it with IC50
value of 17.79 μg/ml. Further study is conducted on ethanol extract 80% of
Scoparia dulcis herbs by using fractination liquid-liquid method and
dichloromethane, ethyl acetate, and butanol as the solvent. The result
showed that the dichloromethane fraction of ethanol extract 80% of
Scoparia dulcis herbs has IC50 value 5.32 ± 0.50 μg/ml. Ethyl acetate,
butanol and water fraction of ethanol extract 80% of Scoparia dulcis herbs
has IC50 value more than 100μg/ml. Dichloromethane fraction of 80%
ethanol extract of Scoparia dulcis showed the highest activity (IC50 5.32 ±
0.50 μg/ml) compare to ethyl acetate, butanol, and water fractions (IC50
more than 100 μg/ml). Dichloromethane fraction of 80% ethanol extract of
Scoparia dulcis has the highest toxicity with CC50 value 23,31 μg/ml and SI
4,38. Ethyl acetate, butanol fraction, and water fraction of ethanol extract
80% of Scoparia dulcis herbs has CC50 value more than 800μg/ml and SI
more than 8. In conclusion, dichloromethane fraction of 80% ethanol extract
of Scoparia dulcis has the highest antiviral activity hepatitis C, but it also
toxic. It has chemical compound such as chlorophyll, terpenoid, and
flavonoid.
Keyword : Scoparia dulcis, extract, fractions, antiHepatitis C virus |
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